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Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men
BACKGROUND: Antioxidant genes, such as superoxide dismutase (SOD), catalase (CAT), and nitric oxide synthase (NOS), play critical roles in spermatogenesis and sperm functions. Polymorphisms of antioxidant genes have been shown to be strongly associated with sperm quality which affects male fertility...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937874/ https://www.ncbi.nlm.nih.gov/pubmed/36592993 http://dx.doi.org/10.1002/jcla.24829 |
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author | Bach, Huy Anh Vu, Phuong Nhung Ma, Thi Huyen Thuong Nguyen, Hai Ha Tran Duc, Phan Bui Minh, Duc Nong, Van Hai Nguyen, Dang Ton |
author_facet | Bach, Huy Anh Vu, Phuong Nhung Ma, Thi Huyen Thuong Nguyen, Hai Ha Tran Duc, Phan Bui Minh, Duc Nong, Van Hai Nguyen, Dang Ton |
author_sort | Bach, Huy Anh |
collection | PubMed |
description | BACKGROUND: Antioxidant genes, such as superoxide dismutase (SOD), catalase (CAT), and nitric oxide synthase (NOS), play critical roles in spermatogenesis and sperm functions. Polymorphisms of antioxidant genes have been shown to be strongly associated with sperm quality which affects male fertility. METHODS: To investigate the association of antioxidant gene polymorphisms to male infertility in Vietnamese men, in this case–control study, using Sanger sequencing, we genotyped four variants SOD1:7958G>A, SOD2:c.47T>C, CAT:‐262C>T, and NOS3:‐786C>T. RESULTS AND CONCLUSIONS: We identified SOD1:7958GA genotype and NOS3:‐786CT genotype in the infertility group were significantly higher than in the control with OR = 2.191 (95% CI: 1.226–3.915, p = 0.004) and OR = 3.135 (95% CI: 1.591–6.180, p < 0.001), respectively. We also detected that the frequency of the SOD2:c.47TC genotype was significantly higher in the male infertility group than in fertile men (OR = 1.941, 95% CI: 1.063–3.595, p = 0.029). Gene–gene interactions between the SNPs of SOD1, SOD2, and CAT might increase the risk of male infertility patients. In particular, patients carrying the SOD1:GA+AA, SOD2:TC+CC, and CAT:CT/TT genotype pattern have an increased risk of male infertility (OR = 7.614, p = 0.007). To our knowledge, this is the first study to evaluate the association between the SOD1:7958G>A polymorphism and male infertility. Further studies with larger sample sizes and more genes are needed to better assess the association between variants of antioxidant genes and male infertility. |
format | Online Article Text |
id | pubmed-9937874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99378742023-02-19 Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men Bach, Huy Anh Vu, Phuong Nhung Ma, Thi Huyen Thuong Nguyen, Hai Ha Tran Duc, Phan Bui Minh, Duc Nong, Van Hai Nguyen, Dang Ton J Clin Lab Anal Research Articles BACKGROUND: Antioxidant genes, such as superoxide dismutase (SOD), catalase (CAT), and nitric oxide synthase (NOS), play critical roles in spermatogenesis and sperm functions. Polymorphisms of antioxidant genes have been shown to be strongly associated with sperm quality which affects male fertility. METHODS: To investigate the association of antioxidant gene polymorphisms to male infertility in Vietnamese men, in this case–control study, using Sanger sequencing, we genotyped four variants SOD1:7958G>A, SOD2:c.47T>C, CAT:‐262C>T, and NOS3:‐786C>T. RESULTS AND CONCLUSIONS: We identified SOD1:7958GA genotype and NOS3:‐786CT genotype in the infertility group were significantly higher than in the control with OR = 2.191 (95% CI: 1.226–3.915, p = 0.004) and OR = 3.135 (95% CI: 1.591–6.180, p < 0.001), respectively. We also detected that the frequency of the SOD2:c.47TC genotype was significantly higher in the male infertility group than in fertile men (OR = 1.941, 95% CI: 1.063–3.595, p = 0.029). Gene–gene interactions between the SNPs of SOD1, SOD2, and CAT might increase the risk of male infertility patients. In particular, patients carrying the SOD1:GA+AA, SOD2:TC+CC, and CAT:CT/TT genotype pattern have an increased risk of male infertility (OR = 7.614, p = 0.007). To our knowledge, this is the first study to evaluate the association between the SOD1:7958G>A polymorphism and male infertility. Further studies with larger sample sizes and more genes are needed to better assess the association between variants of antioxidant genes and male infertility. John Wiley and Sons Inc. 2023-01-02 /pmc/articles/PMC9937874/ /pubmed/36592993 http://dx.doi.org/10.1002/jcla.24829 Text en © 2023 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Bach, Huy Anh Vu, Phuong Nhung Ma, Thi Huyen Thuong Nguyen, Hai Ha Tran Duc, Phan Bui Minh, Duc Nong, Van Hai Nguyen, Dang Ton Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men |
title | Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men |
title_full | Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men |
title_fullStr | Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men |
title_full_unstemmed | Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men |
title_short | Genetic variations of antioxidant genes and their association with male infertility in Vietnamese men |
title_sort | genetic variations of antioxidant genes and their association with male infertility in vietnamese men |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937874/ https://www.ncbi.nlm.nih.gov/pubmed/36592993 http://dx.doi.org/10.1002/jcla.24829 |
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