Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models

We present a protocol to evaluate the impact of senescence secretome on reprogramming to pluripotency using both cellular and mouse models. First, we describe the in vitro reprogramming procedure using conditioned medium derived from senescent cells. Next, to explore the impact of senescence on in v...

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Detalles Bibliográficos
Autores principales: Chantrel, Jérémy, Chen, Cheng, Zhang, Jun, Li, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937951/
https://www.ncbi.nlm.nih.gov/pubmed/36853727
http://dx.doi.org/10.1016/j.xpro.2023.102106
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author Chantrel, Jérémy
Chen, Cheng
Zhang, Jun
Li, Han
author_facet Chantrel, Jérémy
Chen, Cheng
Zhang, Jun
Li, Han
author_sort Chantrel, Jérémy
collection PubMed
description We present a protocol to evaluate the impact of senescence secretome on reprogramming to pluripotency using both cellular and mouse models. First, we describe the in vitro reprogramming procedure using conditioned medium derived from senescent cells. Next, to explore the impact of senescence on in vivo reprogramming, we detail the steps to identify senescent and reprogrammed cells in mouse skeletal muscle, followed by semi-automatic quantification. This protocol can be used to study the effect of paracrine senescence on cellular plasticity. For complete details on the use and execution of this protocol, please refer to von Joest et al. (2022).(1)
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spelling pubmed-99379512023-02-19 Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models Chantrel, Jérémy Chen, Cheng Zhang, Jun Li, Han STAR Protoc Protocol We present a protocol to evaluate the impact of senescence secretome on reprogramming to pluripotency using both cellular and mouse models. First, we describe the in vitro reprogramming procedure using conditioned medium derived from senescent cells. Next, to explore the impact of senescence on in vivo reprogramming, we detail the steps to identify senescent and reprogrammed cells in mouse skeletal muscle, followed by semi-automatic quantification. This protocol can be used to study the effect of paracrine senescence on cellular plasticity. For complete details on the use and execution of this protocol, please refer to von Joest et al. (2022).(1) Elsevier 2023-02-07 /pmc/articles/PMC9937951/ /pubmed/36853727 http://dx.doi.org/10.1016/j.xpro.2023.102106 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Chantrel, Jérémy
Chen, Cheng
Zhang, Jun
Li, Han
Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
title Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
title_full Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
title_fullStr Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
title_full_unstemmed Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
title_short Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
title_sort protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse models
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9937951/
https://www.ncbi.nlm.nih.gov/pubmed/36853727
http://dx.doi.org/10.1016/j.xpro.2023.102106
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