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Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast
Inorganic polyphosphate is a ubiquitous polymer with myriad roles in cell and organismal physiology. Whereas there is evidence for nuclear polyphosphate, its impact on transcriptional regulation in eukaryotes is unkown. Transcriptional profiling of fission yeast cells lacking polyphosphate (via dele...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938405/ https://www.ncbi.nlm.nih.gov/pubmed/36820394 http://dx.doi.org/10.17912/micropub.biology.000744 |
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author | Sanchez, Ana M. Garg, Angad Schwer, Beate Shuman, Stewart |
author_facet | Sanchez, Ana M. Garg, Angad Schwer, Beate Shuman, Stewart |
author_sort | Sanchez, Ana M. |
collection | PubMed |
description | Inorganic polyphosphate is a ubiquitous polymer with myriad roles in cell and organismal physiology. Whereas there is evidence for nuclear polyphosphate, its impact on transcriptional regulation in eukaryotes is unkown. Transcriptional profiling of fission yeast cells lacking polyphosphate (via deletion of the catalytic subunit Vtc4 of the Vtc4/Vtc2 polyphosphate polymerase complex) elicited de-repression of four protein-coding genes located within the right sub-telomeric arm of chromosome I that is known to be transcriptionally silenced by the TORC2 complex. These genes were equally de-repressed in vtc2 ∆ cells and in cells expressing polymerase-dead Vtc4, signifying that polyphosphate synthesis is required for repression of these sub-telomeric genes. |
format | Online Article Text |
id | pubmed-9938405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-99384052023-02-19 Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast Sanchez, Ana M. Garg, Angad Schwer, Beate Shuman, Stewart MicroPubl Biol New Finding Inorganic polyphosphate is a ubiquitous polymer with myriad roles in cell and organismal physiology. Whereas there is evidence for nuclear polyphosphate, its impact on transcriptional regulation in eukaryotes is unkown. Transcriptional profiling of fission yeast cells lacking polyphosphate (via deletion of the catalytic subunit Vtc4 of the Vtc4/Vtc2 polyphosphate polymerase complex) elicited de-repression of four protein-coding genes located within the right sub-telomeric arm of chromosome I that is known to be transcriptionally silenced by the TORC2 complex. These genes were equally de-repressed in vtc2 ∆ cells and in cells expressing polymerase-dead Vtc4, signifying that polyphosphate synthesis is required for repression of these sub-telomeric genes. Caltech Library 2023-02-03 /pmc/articles/PMC9938405/ /pubmed/36820394 http://dx.doi.org/10.17912/micropub.biology.000744 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Sanchez, Ana M. Garg, Angad Schwer, Beate Shuman, Stewart Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
title | Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
title_full | Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
title_fullStr | Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
title_full_unstemmed | Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
title_short | Inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
title_sort | inorganic polyphosphate abets silencing of a sub-telomeric gene cluster in fission yeast |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938405/ https://www.ncbi.nlm.nih.gov/pubmed/36820394 http://dx.doi.org/10.17912/micropub.biology.000744 |
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