A role for ion homeostasis in yeast ionic liquid tolerance

The model yeast Saccharomyces cerevisiae is being developed as a biocatalyst for the conversion of renewable lignocellulosic biomass into biofuels. The ionic liquid 1-ethyl-3-methylimidazolium chloride (EMIMCl) solubilizes lignocellulose for deconstruction into fermentable sugars, but it inhibits ye...

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Detalles Bibliográficos
Autores principales: Liu, Lisa, Ansari, Rahim U., Vang-Smith, Maikayeng, Hittinger, Chris Todd, Sato, Trey K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938406/
https://www.ncbi.nlm.nih.gov/pubmed/36820393
http://dx.doi.org/10.17912/micropub.biology.000718
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author Liu, Lisa
Ansari, Rahim U.
Vang-Smith, Maikayeng
Hittinger, Chris Todd
Sato, Trey K.
author_facet Liu, Lisa
Ansari, Rahim U.
Vang-Smith, Maikayeng
Hittinger, Chris Todd
Sato, Trey K.
author_sort Liu, Lisa
collection PubMed
description The model yeast Saccharomyces cerevisiae is being developed as a biocatalyst for the conversion of renewable lignocellulosic biomass into biofuels. The ionic liquid 1-ethyl-3-methylimidazolium chloride (EMIMCl) solubilizes lignocellulose for deconstruction into fermentable sugars, but it inhibits yeast fermentation. EMIMCl tolerance is mediated by the efflux pump Sge1p and uncharacterized protein Ilt1p. Through genetic investigation, we found that disruption of ion homeostasis through mutations in genes encoding the Trk1p potassium transporter and its protein kinase regulators, Sat4p and Hal5p, causes EMIMCl sensitivity. These results suggest that maintenance of ion homeostasis is important for tolerance to EMIMCl.
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spelling pubmed-99384062023-02-19 A role for ion homeostasis in yeast ionic liquid tolerance Liu, Lisa Ansari, Rahim U. Vang-Smith, Maikayeng Hittinger, Chris Todd Sato, Trey K. MicroPubl Biol New Finding The model yeast Saccharomyces cerevisiae is being developed as a biocatalyst for the conversion of renewable lignocellulosic biomass into biofuels. The ionic liquid 1-ethyl-3-methylimidazolium chloride (EMIMCl) solubilizes lignocellulose for deconstruction into fermentable sugars, but it inhibits yeast fermentation. EMIMCl tolerance is mediated by the efflux pump Sge1p and uncharacterized protein Ilt1p. Through genetic investigation, we found that disruption of ion homeostasis through mutations in genes encoding the Trk1p potassium transporter and its protein kinase regulators, Sat4p and Hal5p, causes EMIMCl sensitivity. These results suggest that maintenance of ion homeostasis is important for tolerance to EMIMCl. Caltech Library 2023-02-03 /pmc/articles/PMC9938406/ /pubmed/36820393 http://dx.doi.org/10.17912/micropub.biology.000718 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Liu, Lisa
Ansari, Rahim U.
Vang-Smith, Maikayeng
Hittinger, Chris Todd
Sato, Trey K.
A role for ion homeostasis in yeast ionic liquid tolerance
title A role for ion homeostasis in yeast ionic liquid tolerance
title_full A role for ion homeostasis in yeast ionic liquid tolerance
title_fullStr A role for ion homeostasis in yeast ionic liquid tolerance
title_full_unstemmed A role for ion homeostasis in yeast ionic liquid tolerance
title_short A role for ion homeostasis in yeast ionic liquid tolerance
title_sort role for ion homeostasis in yeast ionic liquid tolerance
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938406/
https://www.ncbi.nlm.nih.gov/pubmed/36820393
http://dx.doi.org/10.17912/micropub.biology.000718
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