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FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia

INTRODUCTION: Phagocytosis of autoantibody-sensitized coated platelets through Fc gamma receptors on phagocytic cells is an important mechanism of thrombocytopenia in primary immune thrombocytopenia (ITP). OBJECTIVE: We aimed to investigate the contribution of the FcγRIIa and FcγRIIIa genes polymorp...

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Autores principales: Zakaria, Marwa, Al-Akhras, Ahmed, Hassan, Tamer, Sherief, Laila, Magdy, Wessam, Raafat, Nermin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Hematologia e Hemoterapia 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938460/
https://www.ncbi.nlm.nih.gov/pubmed/34266810
http://dx.doi.org/10.1016/j.htct.2021.05.007
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author Zakaria, Marwa
Al-Akhras, Ahmed
Hassan, Tamer
Sherief, Laila
Magdy, Wessam
Raafat, Nermin
author_facet Zakaria, Marwa
Al-Akhras, Ahmed
Hassan, Tamer
Sherief, Laila
Magdy, Wessam
Raafat, Nermin
author_sort Zakaria, Marwa
collection PubMed
description INTRODUCTION: Phagocytosis of autoantibody-sensitized coated platelets through Fc gamma receptors on phagocytic cells is an important mechanism of thrombocytopenia in primary immune thrombocytopenia (ITP). OBJECTIVE: We aimed to investigate the contribution of the FcγRIIa and FcγRIIIa genes polymorphism to the risk of ITP and their association with disease characteristics in Egyptian children. METHODS: A case control study was conducted on eighty children with primary ITP and eighty age and sex healthy matched subjects as a control group. The FcγRIIa and FcγRIIIa genes polymorphism was detected using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: We found that the FcγRIIa‐131H and ‐131R allele frequencies were 51.3 % and 48.7%, respectively, in children with ITP, versus 75% and 25%, respectively, in controls (p = 0.002). The compound heterozygous HR genotype was significantly higher in ITP patients (p < 0.05). The FcγRIIIa-158F and ‐158V allele frequencies were 46.3% and 53.7%, respectively, in children with ITP, versus 70% and 30%, respectively, in controls (p = 0.002). The compound heterozygous VF genotype was significantly higher in ITP patients (p < 0.05). The combined HR/FV genotype was 47.5% in ITP patients, versus 10% in controls (p < 0.001). No significant difference was found between children with newly diagnosed ITP and those who developed chronic ITP, regarding the frequency distribution of the FcγRIIa and FcγRIIIa alleles and genotypes (p > 0.05). CONCLUSION: There is a possible association of the FcγRIIa and FcγRIIIa genes polymorphism with the risk for, and genetic susceptibility to ITP in Egyptian children, but large-scale studies are still needed to support our findings.
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spelling pubmed-99384602023-02-19 FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia Zakaria, Marwa Al-Akhras, Ahmed Hassan, Tamer Sherief, Laila Magdy, Wessam Raafat, Nermin Hematol Transfus Cell Ther Original Article INTRODUCTION: Phagocytosis of autoantibody-sensitized coated platelets through Fc gamma receptors on phagocytic cells is an important mechanism of thrombocytopenia in primary immune thrombocytopenia (ITP). OBJECTIVE: We aimed to investigate the contribution of the FcγRIIa and FcγRIIIa genes polymorphism to the risk of ITP and their association with disease characteristics in Egyptian children. METHODS: A case control study was conducted on eighty children with primary ITP and eighty age and sex healthy matched subjects as a control group. The FcγRIIa and FcγRIIIa genes polymorphism was detected using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: We found that the FcγRIIa‐131H and ‐131R allele frequencies were 51.3 % and 48.7%, respectively, in children with ITP, versus 75% and 25%, respectively, in controls (p = 0.002). The compound heterozygous HR genotype was significantly higher in ITP patients (p < 0.05). The FcγRIIIa-158F and ‐158V allele frequencies were 46.3% and 53.7%, respectively, in children with ITP, versus 70% and 30%, respectively, in controls (p = 0.002). The compound heterozygous VF genotype was significantly higher in ITP patients (p < 0.05). The combined HR/FV genotype was 47.5% in ITP patients, versus 10% in controls (p < 0.001). No significant difference was found between children with newly diagnosed ITP and those who developed chronic ITP, regarding the frequency distribution of the FcγRIIa and FcγRIIIa alleles and genotypes (p > 0.05). CONCLUSION: There is a possible association of the FcγRIIa and FcγRIIIa genes polymorphism with the risk for, and genetic susceptibility to ITP in Egyptian children, but large-scale studies are still needed to support our findings. Sociedade Brasileira de Hematologia e Hemoterapia 2023 2021-07-06 /pmc/articles/PMC9938460/ /pubmed/34266810 http://dx.doi.org/10.1016/j.htct.2021.05.007 Text en © 2021 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier España, S.L.U. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Zakaria, Marwa
Al-Akhras, Ahmed
Hassan, Tamer
Sherief, Laila
Magdy, Wessam
Raafat, Nermin
FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia
title FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia
title_full FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia
title_fullStr FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia
title_full_unstemmed FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia
title_short FcγRIIa and FcγRIIIa genes polymorphism in Egyptian children with primary immune thrombocytopenia
title_sort fcγriia and fcγriiia genes polymorphism in egyptian children with primary immune thrombocytopenia
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938460/
https://www.ncbi.nlm.nih.gov/pubmed/34266810
http://dx.doi.org/10.1016/j.htct.2021.05.007
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