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Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions
BACKGROUND: Early detection and prevention of precancerous lesions can significantly reduce the morbidity and mortality of colorectal cancer (CRC). Here, we developed new candidate CpG site biomarkers for CRC and evaluated the diagnostic value of their expression in blood and stool samples of CRC an...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938553/ https://www.ncbi.nlm.nih.gov/pubmed/36803423 http://dx.doi.org/10.1186/s13148-023-01443-7 |
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author | Shen, Yuguang Wang, Dongyang Yuan, Tianli Fang, Hongsheng Zhu, Chen Qin, Juan Xu, Xiaojing Zhang, Cheng Liu, Jiahua Zhang, Yuanruohan Wen, Zhoujin Tang, Jian Wang, Zheng |
author_facet | Shen, Yuguang Wang, Dongyang Yuan, Tianli Fang, Hongsheng Zhu, Chen Qin, Juan Xu, Xiaojing Zhang, Cheng Liu, Jiahua Zhang, Yuanruohan Wen, Zhoujin Tang, Jian Wang, Zheng |
author_sort | Shen, Yuguang |
collection | PubMed |
description | BACKGROUND: Early detection and prevention of precancerous lesions can significantly reduce the morbidity and mortality of colorectal cancer (CRC). Here, we developed new candidate CpG site biomarkers for CRC and evaluated the diagnostic value of their expression in blood and stool samples of CRC and precancerous lesions. METHODS: We analyzed 76 pairs of CRC and adjacent normal tissue samples, 348 stool samples, and 136 blood samples. Candidate biomarkers for CRC were screened using a bioinformatics database and identified using a quantitative methylation-specific PCR method. The methylation levels of the candidate biomarkers were validated using blood and stool samples. The divided stool samples were used to construct and validate a combined diagnostic model and to analyze the independent or combined diagnostic value of candidate biomarkers in stool samples of CRC and precancerous lesions. RESULTS: Two candidate CpG site biomarkers for CRC, cg13096260 and cg12993163, were identified. Although both biomarkers demonstrated diagnostic performance to a certain extent when using blood samples, they showed better diagnostic value for different stages of CRC and AA with stool samples. CONCLUSIONS: cg13096260 and cg12993163 detection in stool samples could be a promising approach for screening and early diagnosis of CRC and precancerous lesions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-023-01443-7. |
format | Online Article Text |
id | pubmed-9938553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-99385532023-02-19 Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions Shen, Yuguang Wang, Dongyang Yuan, Tianli Fang, Hongsheng Zhu, Chen Qin, Juan Xu, Xiaojing Zhang, Cheng Liu, Jiahua Zhang, Yuanruohan Wen, Zhoujin Tang, Jian Wang, Zheng Clin Epigenetics Research BACKGROUND: Early detection and prevention of precancerous lesions can significantly reduce the morbidity and mortality of colorectal cancer (CRC). Here, we developed new candidate CpG site biomarkers for CRC and evaluated the diagnostic value of their expression in blood and stool samples of CRC and precancerous lesions. METHODS: We analyzed 76 pairs of CRC and adjacent normal tissue samples, 348 stool samples, and 136 blood samples. Candidate biomarkers for CRC were screened using a bioinformatics database and identified using a quantitative methylation-specific PCR method. The methylation levels of the candidate biomarkers were validated using blood and stool samples. The divided stool samples were used to construct and validate a combined diagnostic model and to analyze the independent or combined diagnostic value of candidate biomarkers in stool samples of CRC and precancerous lesions. RESULTS: Two candidate CpG site biomarkers for CRC, cg13096260 and cg12993163, were identified. Although both biomarkers demonstrated diagnostic performance to a certain extent when using blood samples, they showed better diagnostic value for different stages of CRC and AA with stool samples. CONCLUSIONS: cg13096260 and cg12993163 detection in stool samples could be a promising approach for screening and early diagnosis of CRC and precancerous lesions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13148-023-01443-7. BioMed Central 2023-02-17 /pmc/articles/PMC9938553/ /pubmed/36803423 http://dx.doi.org/10.1186/s13148-023-01443-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Shen, Yuguang Wang, Dongyang Yuan, Tianli Fang, Hongsheng Zhu, Chen Qin, Juan Xu, Xiaojing Zhang, Cheng Liu, Jiahua Zhang, Yuanruohan Wen, Zhoujin Tang, Jian Wang, Zheng Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
title | Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
title_full | Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
title_fullStr | Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
title_full_unstemmed | Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
title_short | Novel DNA methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
title_sort | novel dna methylation biomarkers in stool and blood for early detection of colorectal cancer and precancerous lesions |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9938553/ https://www.ncbi.nlm.nih.gov/pubmed/36803423 http://dx.doi.org/10.1186/s13148-023-01443-7 |
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