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BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression

The SWI/SNF complex remodels chromatin in an ATP-dependent manner through the subunits BRG1 and BRM. Chromatin remodeling alters nucleosome structure to change gene expression; however, aberrant remodeling can result in cancer. We identified BCL7 proteins as critical SWI/SNF members that drive BRG1-...

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Detalles Bibliográficos
Autores principales: Dietrich, Nicholas, Trotter, Kevin, Ward, James M, Archer, Trevor K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939006/
https://www.ncbi.nlm.nih.gov/pubmed/36801810
http://dx.doi.org/10.26508/lsa.202201770
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author Dietrich, Nicholas
Trotter, Kevin
Ward, James M
Archer, Trevor K
author_facet Dietrich, Nicholas
Trotter, Kevin
Ward, James M
Archer, Trevor K
author_sort Dietrich, Nicholas
collection PubMed
description The SWI/SNF complex remodels chromatin in an ATP-dependent manner through the subunits BRG1 and BRM. Chromatin remodeling alters nucleosome structure to change gene expression; however, aberrant remodeling can result in cancer. We identified BCL7 proteins as critical SWI/SNF members that drive BRG1-dependent gene expression changes. BCL7s have been implicated in B-cell lymphoma, but characterization of their functional role within the SWI/SNF complex has been limited. This study implicates their function alongside BRG1 to drive large-scale changes in gene expression. Mechanistically, the BCL7 proteins bind to the HSA domain of BRG1 and require this domain for binding to chromatin. BRG1 proteins without the HSA domain fail to interact with the BCL7 proteins and have severely reduced chromatin remodeling activity. These results link the HSA domain and the formation of a functional SWI/SNF remodeling complex through the interaction with BCL7 proteins. These data highlight the importance of correct formation of the SWI/SNF complex to drive critical biological functions, as losses of individual accessory members or protein domains can cause loss of complex function.
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spelling pubmed-99390062023-02-20 BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression Dietrich, Nicholas Trotter, Kevin Ward, James M Archer, Trevor K Life Sci Alliance Research Articles The SWI/SNF complex remodels chromatin in an ATP-dependent manner through the subunits BRG1 and BRM. Chromatin remodeling alters nucleosome structure to change gene expression; however, aberrant remodeling can result in cancer. We identified BCL7 proteins as critical SWI/SNF members that drive BRG1-dependent gene expression changes. BCL7s have been implicated in B-cell lymphoma, but characterization of their functional role within the SWI/SNF complex has been limited. This study implicates their function alongside BRG1 to drive large-scale changes in gene expression. Mechanistically, the BCL7 proteins bind to the HSA domain of BRG1 and require this domain for binding to chromatin. BRG1 proteins without the HSA domain fail to interact with the BCL7 proteins and have severely reduced chromatin remodeling activity. These results link the HSA domain and the formation of a functional SWI/SNF remodeling complex through the interaction with BCL7 proteins. These data highlight the importance of correct formation of the SWI/SNF complex to drive critical biological functions, as losses of individual accessory members or protein domains can cause loss of complex function. Life Science Alliance LLC 2023-02-17 /pmc/articles/PMC9939006/ /pubmed/36801810 http://dx.doi.org/10.26508/lsa.202201770 Text en © 2023 Dietrich et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Dietrich, Nicholas
Trotter, Kevin
Ward, James M
Archer, Trevor K
BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
title BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
title_full BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
title_fullStr BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
title_full_unstemmed BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
title_short BRG1 HSA domain interactions with BCL7 proteins are critical for remodeling and gene expression
title_sort brg1 hsa domain interactions with bcl7 proteins are critical for remodeling and gene expression
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939006/
https://www.ncbi.nlm.nih.gov/pubmed/36801810
http://dx.doi.org/10.26508/lsa.202201770
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