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Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats

We examined various methods to enhance the accessibility of intracytoplasmic sperm injection (ICSI) technology to more users by making the technique easier, more efficient, and practical. First, the methods for artificially removing the mouse sperm tail were evaluated. Trypsin treatment was found to...

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Detalles Bibliográficos
Autores principales:	TORIKAI, Kohei, SHIMIZU, Kazuma, NAGATOMO, Hiroaki, KASAI, Mariko, KATO-ITOH, Megumi, KAMADA, Yuko, SHIBASAKI, Ikue, JEON, Hyojung, KIKUCHI, Riko, WAKAYAMA, Sayaka, SUCHY, Fabian, NAKAUCHI, Hiromitsu, WAKAYAMA, Teruhiko, MIZUTANI, Eiji
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Society for Reproduction and Development 2022
Materias:	Technology Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939287/ https://www.ncbi.nlm.nih.gov/pubmed/36529517 http://dx.doi.org/10.1262/jrd.2022-065

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