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A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells

CD4(+) T cells are critical to the immune system and perform multiple functions; therefore, their identification and characterization are crucial to better understanding the immune system in both health and disease states. However, current methods rarely preserve their ex vivo phenotype, thus limiti...

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Autores principales: Vyasamneni, Rohit, Kohler, Victoria, Karki, Binisha, Mahimkar, Gauri, Esaulova, Ekaterina, McGee, Jonathan, Kallin, Daniel, Sheen, Joong Hyuk, Harjanto, Dewi, Kirsch, Miles, Poran, Asaf, Dong, Jesse, Srinivasan, Lakshmi, Gaynor, Richard B., Bushway, Meghan E., Srouji, John R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939426/
https://www.ncbi.nlm.nih.gov/pubmed/36814840
http://dx.doi.org/10.1016/j.crmeth.2022.100388
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author Vyasamneni, Rohit
Kohler, Victoria
Karki, Binisha
Mahimkar, Gauri
Esaulova, Ekaterina
McGee, Jonathan
Kallin, Daniel
Sheen, Joong Hyuk
Harjanto, Dewi
Kirsch, Miles
Poran, Asaf
Dong, Jesse
Srinivasan, Lakshmi
Gaynor, Richard B.
Bushway, Meghan E.
Srouji, John R.
author_facet Vyasamneni, Rohit
Kohler, Victoria
Karki, Binisha
Mahimkar, Gauri
Esaulova, Ekaterina
McGee, Jonathan
Kallin, Daniel
Sheen, Joong Hyuk
Harjanto, Dewi
Kirsch, Miles
Poran, Asaf
Dong, Jesse
Srinivasan, Lakshmi
Gaynor, Richard B.
Bushway, Meghan E.
Srouji, John R.
author_sort Vyasamneni, Rohit
collection PubMed
description CD4(+) T cells are critical to the immune system and perform multiple functions; therefore, their identification and characterization are crucial to better understanding the immune system in both health and disease states. However, current methods rarely preserve their ex vivo phenotype, thus limiting our understanding of their in vivo functions. Here we introduce a flexible, rapid, and robust platform for ex vivo CD4(+) T cell identification. By combining MHCII allele purification, allele-independent peptide loading, and multiplexed flow cytometry technologies, we can enable high-throughput personalized CD4(+) T cell identification, immunophenotyping, and sorting. Using this platform in combination with single-cell sorting and multimodal analyses, we identified and characterized antigen-specific CD4(+) T cells relevant to COVID-19 and cancer neoantigen immunotherapy. Overall, our platform can be used to detect and characterize CD4(+) T cells across multiple diseases, with potential to guide CD4(+) T cell epitope design for any disease-specific immunization strategy.
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spelling pubmed-99394262023-02-21 A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells Vyasamneni, Rohit Kohler, Victoria Karki, Binisha Mahimkar, Gauri Esaulova, Ekaterina McGee, Jonathan Kallin, Daniel Sheen, Joong Hyuk Harjanto, Dewi Kirsch, Miles Poran, Asaf Dong, Jesse Srinivasan, Lakshmi Gaynor, Richard B. Bushway, Meghan E. Srouji, John R. Cell Rep Methods Article CD4(+) T cells are critical to the immune system and perform multiple functions; therefore, their identification and characterization are crucial to better understanding the immune system in both health and disease states. However, current methods rarely preserve their ex vivo phenotype, thus limiting our understanding of their in vivo functions. Here we introduce a flexible, rapid, and robust platform for ex vivo CD4(+) T cell identification. By combining MHCII allele purification, allele-independent peptide loading, and multiplexed flow cytometry technologies, we can enable high-throughput personalized CD4(+) T cell identification, immunophenotyping, and sorting. Using this platform in combination with single-cell sorting and multimodal analyses, we identified and characterized antigen-specific CD4(+) T cells relevant to COVID-19 and cancer neoantigen immunotherapy. Overall, our platform can be used to detect and characterize CD4(+) T cells across multiple diseases, with potential to guide CD4(+) T cell epitope design for any disease-specific immunization strategy. Elsevier 2023-01-13 /pmc/articles/PMC9939426/ /pubmed/36814840 http://dx.doi.org/10.1016/j.crmeth.2022.100388 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Vyasamneni, Rohit
Kohler, Victoria
Karki, Binisha
Mahimkar, Gauri
Esaulova, Ekaterina
McGee, Jonathan
Kallin, Daniel
Sheen, Joong Hyuk
Harjanto, Dewi
Kirsch, Miles
Poran, Asaf
Dong, Jesse
Srinivasan, Lakshmi
Gaynor, Richard B.
Bushway, Meghan E.
Srouji, John R.
A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells
title A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells
title_full A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells
title_fullStr A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells
title_full_unstemmed A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells
title_short A universal MHCII technology platform to characterize antigen-specific CD4(+) T cells
title_sort universal mhcii technology platform to characterize antigen-specific cd4(+) t cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939426/
https://www.ncbi.nlm.nih.gov/pubmed/36814840
http://dx.doi.org/10.1016/j.crmeth.2022.100388
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