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Comprehensive evaluation of shotgun metagenomics, amplicon sequencing, and harmonization of these platforms for epidemiological studies

In a large cohort of 1,772 participants from the Hispanic Community Health Study/Study of Latinos with overlapping 16SV4 rRNA gene (bacterial amplicon), ITS1 (fungal amplicon), and shotgun sequencing data, we demonstrate that 16SV4 amplicon sequencing and shotgun metagenomics offer the same level of...

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Detalles Bibliográficos
Autores principales: Usyk, Mykhaylo, Peters, Brandilyn A., Karthikeyan, Smruthi, McDonald, Daniel, Sollecito, Christopher C., Vazquez-Baeza, Yoshiki, Shaffer, Justin P., Gellman, Marc D., Talavera, Gregory A., Daviglus, Martha L., Thyagarajan, Bharat, Knight, Rob, Qi, Qibin, Kaplan, Robert, Burk, Robert D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939430/
https://www.ncbi.nlm.nih.gov/pubmed/36814836
http://dx.doi.org/10.1016/j.crmeth.2022.100391