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Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells

To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC(50)) were detected using the CCK-8 method, and the...

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Autores principales: Feng, Duo, Zhou, Shi-qi, Zhou, Ya-xi, Jiang, Yong-jun, Sun, Qiao-di, Song, Wei, Cui, Qian-qian, Yan, Wen-jie, Wang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939456/
https://www.ncbi.nlm.nih.gov/pubmed/36814512
http://dx.doi.org/10.3389/fnut.2023.1117364
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author Feng, Duo
Zhou, Shi-qi
Zhou, Ya-xi
Jiang, Yong-jun
Sun, Qiao-di
Song, Wei
Cui, Qian-qian
Yan, Wen-jie
Wang, Jing
author_facet Feng, Duo
Zhou, Shi-qi
Zhou, Ya-xi
Jiang, Yong-jun
Sun, Qiao-di
Song, Wei
Cui, Qian-qian
Yan, Wen-jie
Wang, Jing
author_sort Feng, Duo
collection PubMed
description To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC(50)) were detected using the CCK-8 method, and the level of reactive oxygen species (ROS) was detected by using a DCFH-DA fluorescence probe. Finally, a Seahorse XFe24 energy analyzer (Agilent, United States) was used to detect cell mitochondrial pressure and glycolytic pressure. The results showed that TG could reduce the survival rate of HepG2 cells and that the IC(50) level was 35.28 μg/ml. With increasing TG concentration, the level of ROS showed a concentration-dependent upward trend. Energy metabolism showed that each dose group of TG could significantly decline the mitochondrial respiratory and glycolytic functions of HepG2 cells. In conclusion, TG could significantly inhibit the mitochondrial respiration and glycolysis functions of HepG2 cells, increase the level of ROS, and inhibit cell proliferation. Thus, this experiment pointed out that Cistanche deserticola can be used as a source of anti-cancer foods or drugs in the future. However, further studies on its mechanisms and clinical applications are needed.
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spelling pubmed-99394562023-02-21 Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells Feng, Duo Zhou, Shi-qi Zhou, Ya-xi Jiang, Yong-jun Sun, Qiao-di Song, Wei Cui, Qian-qian Yan, Wen-jie Wang, Jing Front Nutr Nutrition To study the anti-tumor effect of Cistanche deserticola Y. Ma, HepG2 cells were treated with 0, 3.5, 10.5, 21, 31.5, and 42 μg/ml of total glycosides (TG) from Cistanche deserticola. The HepG2 cell survival rate and 50% inhibition concentration (IC(50)) were detected using the CCK-8 method, and the level of reactive oxygen species (ROS) was detected by using a DCFH-DA fluorescence probe. Finally, a Seahorse XFe24 energy analyzer (Agilent, United States) was used to detect cell mitochondrial pressure and glycolytic pressure. The results showed that TG could reduce the survival rate of HepG2 cells and that the IC(50) level was 35.28 μg/ml. With increasing TG concentration, the level of ROS showed a concentration-dependent upward trend. Energy metabolism showed that each dose group of TG could significantly decline the mitochondrial respiratory and glycolytic functions of HepG2 cells. In conclusion, TG could significantly inhibit the mitochondrial respiration and glycolysis functions of HepG2 cells, increase the level of ROS, and inhibit cell proliferation. Thus, this experiment pointed out that Cistanche deserticola can be used as a source of anti-cancer foods or drugs in the future. However, further studies on its mechanisms and clinical applications are needed. Frontiers Media S.A. 2023-02-06 /pmc/articles/PMC9939456/ /pubmed/36814512 http://dx.doi.org/10.3389/fnut.2023.1117364 Text en Copyright © 2023 Feng, Zhou, Zhou, Jiang, Sun, Song, Cui, Yan and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Feng, Duo
Zhou, Shi-qi
Zhou, Ya-xi
Jiang, Yong-jun
Sun, Qiao-di
Song, Wei
Cui, Qian-qian
Yan, Wen-jie
Wang, Jing
Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
title Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
title_full Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
title_fullStr Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
title_full_unstemmed Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
title_short Effect of total glycosides of Cistanche deserticola on the energy metabolism of human HepG2 cells
title_sort effect of total glycosides of cistanche deserticola on the energy metabolism of human hepg2 cells
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939456/
https://www.ncbi.nlm.nih.gov/pubmed/36814512
http://dx.doi.org/10.3389/fnut.2023.1117364
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