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The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity

Chickpea fields in Saskatchewan, one of the three Canadian prairie provinces, have suffered from major health issues since 2019, but no definitive cause has been determined. Field surveys were conducted in Saskatchewan in 2020 and 2021 in order to develop a better understanding of root rot pathogens...

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Autores principales: Armstrong-Cho, Cheryl, Sivachandra Kumar, Nimllash Thangam, Kaur, Ramanpreet, Banniza, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939516/
https://www.ncbi.nlm.nih.gov/pubmed/36814759
http://dx.doi.org/10.3389/fpls.2023.1117788
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author Armstrong-Cho, Cheryl
Sivachandra Kumar, Nimllash Thangam
Kaur, Ramanpreet
Banniza, Sabine
author_facet Armstrong-Cho, Cheryl
Sivachandra Kumar, Nimllash Thangam
Kaur, Ramanpreet
Banniza, Sabine
author_sort Armstrong-Cho, Cheryl
collection PubMed
description Chickpea fields in Saskatchewan, one of the three Canadian prairie provinces, have suffered from major health issues since 2019, but no definitive cause has been determined. Field surveys were conducted in Saskatchewan in 2020 and 2021 in order to develop a better understanding of root rot pathogens associated with chickpea. Root samples were analyzed for the presence of 11 potential chickpea root rot pathogens using end-point PCR. Fusarium redolens, F. solani and F. avenaceum were the most prevalent pathogen species detected in both survey years. The cause of Fusarium wilt in chickpea, F. oxysporum f. sp. ciceris, was not detected in either year, nor were Phytophthora spp. and Verticillium albo-atrum. Berkeleyomyces sp. was detected in one field in each year, and Verticillium dahliae was detected in several fields sampled in 2021. These two pathogens have not been reported previously on chickpea in Saskatchewan. The prevalence of Fusarium species obtained from 2021 root isolations was similar to that determined by molecular tests, with frequent isolation of F. redolens, F. oxysporum, F. avenaceum and F. solani. A series of indoor pathogenicity testing compared root disease severity caused by a selection of 16 isolates of six Fusarium species and single isolates of V. dahliae, Berkeleyomyces sp. and Macrophomina phaseolina. Results showed that select isolates of F. avenaceum were the most aggressive of the Fusarium isolates on chickpea. Despite relatively low inoculum density, a highly aggressive isolate of F. avenaceum caused severe stunting and more root rot symptoms than single isolates of V. dahliae, Berkeleyomyces sp. and M. phaseolina under the test conditions.
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spelling pubmed-99395162023-02-21 The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity Armstrong-Cho, Cheryl Sivachandra Kumar, Nimllash Thangam Kaur, Ramanpreet Banniza, Sabine Front Plant Sci Plant Science Chickpea fields in Saskatchewan, one of the three Canadian prairie provinces, have suffered from major health issues since 2019, but no definitive cause has been determined. Field surveys were conducted in Saskatchewan in 2020 and 2021 in order to develop a better understanding of root rot pathogens associated with chickpea. Root samples were analyzed for the presence of 11 potential chickpea root rot pathogens using end-point PCR. Fusarium redolens, F. solani and F. avenaceum were the most prevalent pathogen species detected in both survey years. The cause of Fusarium wilt in chickpea, F. oxysporum f. sp. ciceris, was not detected in either year, nor were Phytophthora spp. and Verticillium albo-atrum. Berkeleyomyces sp. was detected in one field in each year, and Verticillium dahliae was detected in several fields sampled in 2021. These two pathogens have not been reported previously on chickpea in Saskatchewan. The prevalence of Fusarium species obtained from 2021 root isolations was similar to that determined by molecular tests, with frequent isolation of F. redolens, F. oxysporum, F. avenaceum and F. solani. A series of indoor pathogenicity testing compared root disease severity caused by a selection of 16 isolates of six Fusarium species and single isolates of V. dahliae, Berkeleyomyces sp. and Macrophomina phaseolina. Results showed that select isolates of F. avenaceum were the most aggressive of the Fusarium isolates on chickpea. Despite relatively low inoculum density, a highly aggressive isolate of F. avenaceum caused severe stunting and more root rot symptoms than single isolates of V. dahliae, Berkeleyomyces sp. and M. phaseolina under the test conditions. Frontiers Media S.A. 2023-02-06 /pmc/articles/PMC9939516/ /pubmed/36814759 http://dx.doi.org/10.3389/fpls.2023.1117788 Text en Copyright © 2023 Armstrong-Cho, Sivachandra Kumar, Kaur and Banniza https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Armstrong-Cho, Cheryl
Sivachandra Kumar, Nimllash Thangam
Kaur, Ramanpreet
Banniza, Sabine
The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity
title The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity
title_full The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity
title_fullStr The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity
title_full_unstemmed The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity
title_short The chickpea root rot complex in Saskatchewan, Canada- detection of emerging pathogens and their relative pathogenicity
title_sort chickpea root rot complex in saskatchewan, canada- detection of emerging pathogens and their relative pathogenicity
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939516/
https://www.ncbi.nlm.nih.gov/pubmed/36814759
http://dx.doi.org/10.3389/fpls.2023.1117788
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