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Transcription factor-based biosensors for screening and dynamic regulation

Advances in synthetic biology and genetic engineering are bringing into the spotlight a wide range of bio-based applications that demand better sensing and control of biological behaviours. Transcription factor (TF)-based biosensors are promising tools that can be used to detect several types of che...

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Autores principales: Tellechea-Luzardo, Jonathan, Stiebritz, Martin T., Carbonell, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939652/
https://www.ncbi.nlm.nih.gov/pubmed/36814719
http://dx.doi.org/10.3389/fbioe.2023.1118702
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author Tellechea-Luzardo, Jonathan
Stiebritz, Martin T.
Carbonell, Pablo
author_facet Tellechea-Luzardo, Jonathan
Stiebritz, Martin T.
Carbonell, Pablo
author_sort Tellechea-Luzardo, Jonathan
collection PubMed
description Advances in synthetic biology and genetic engineering are bringing into the spotlight a wide range of bio-based applications that demand better sensing and control of biological behaviours. Transcription factor (TF)-based biosensors are promising tools that can be used to detect several types of chemical compounds and elicit a response according to the desired application. However, the wider use of this type of device is still hindered by several challenges, which can be addressed by increasing the current metabolite-activated transcription factor knowledge base, developing better methods to identify new transcription factors, and improving the overall workflow for the design of novel biosensor circuits. These improvements are particularly important in the bioproduction field, where researchers need better biosensor-based approaches for screening production-strains and precise dynamic regulation strategies. In this work, we summarize what is currently known about transcription factor-based biosensors, discuss recent experimental and computational approaches targeted at their modification and improvement, and suggest possible future research directions based on two applications: bioproduction screening and dynamic regulation of genetic circuits.
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spelling pubmed-99396522023-02-21 Transcription factor-based biosensors for screening and dynamic regulation Tellechea-Luzardo, Jonathan Stiebritz, Martin T. Carbonell, Pablo Front Bioeng Biotechnol Bioengineering and Biotechnology Advances in synthetic biology and genetic engineering are bringing into the spotlight a wide range of bio-based applications that demand better sensing and control of biological behaviours. Transcription factor (TF)-based biosensors are promising tools that can be used to detect several types of chemical compounds and elicit a response according to the desired application. However, the wider use of this type of device is still hindered by several challenges, which can be addressed by increasing the current metabolite-activated transcription factor knowledge base, developing better methods to identify new transcription factors, and improving the overall workflow for the design of novel biosensor circuits. These improvements are particularly important in the bioproduction field, where researchers need better biosensor-based approaches for screening production-strains and precise dynamic regulation strategies. In this work, we summarize what is currently known about transcription factor-based biosensors, discuss recent experimental and computational approaches targeted at their modification and improvement, and suggest possible future research directions based on two applications: bioproduction screening and dynamic regulation of genetic circuits. Frontiers Media S.A. 2023-02-06 /pmc/articles/PMC9939652/ /pubmed/36814719 http://dx.doi.org/10.3389/fbioe.2023.1118702 Text en Copyright © 2023 Tellechea-Luzardo, Stiebritz and Carbonell. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Tellechea-Luzardo, Jonathan
Stiebritz, Martin T.
Carbonell, Pablo
Transcription factor-based biosensors for screening and dynamic regulation
title Transcription factor-based biosensors for screening and dynamic regulation
title_full Transcription factor-based biosensors for screening and dynamic regulation
title_fullStr Transcription factor-based biosensors for screening and dynamic regulation
title_full_unstemmed Transcription factor-based biosensors for screening and dynamic regulation
title_short Transcription factor-based biosensors for screening and dynamic regulation
title_sort transcription factor-based biosensors for screening and dynamic regulation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9939652/
https://www.ncbi.nlm.nih.gov/pubmed/36814719
http://dx.doi.org/10.3389/fbioe.2023.1118702
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