Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing

The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 regi...

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Detalles Bibliográficos
Autores principales: Ding, F., Krasilnikova, A. A., Leontieva, M. R., Stoyanova, L. G., Netrusov, A. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2023
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940072/
https://www.ncbi.nlm.nih.gov/pubmed/36843649
http://dx.doi.org/10.3103/S0096392522040010
Descripción
Sumario:The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from different regions are lactic acid bacteria (LAB; lactobacilli, lactococci, and Leuconostoc spp. in different ratios) and different types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.

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