Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing

The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 regi...

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Autores principales: Ding, F., Krasilnikova, A. A., Leontieva, M. R., Stoyanova, L. G., Netrusov, A. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pleiades Publishing 2023
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940072/
https://www.ncbi.nlm.nih.gov/pubmed/36843649
http://dx.doi.org/10.3103/S0096392522040010
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author Ding, F.
Krasilnikova, A. A.
Leontieva, M. R.
Stoyanova, L. G.
Netrusov, A. I.
author_facet Ding, F.
Krasilnikova, A. A.
Leontieva, M. R.
Stoyanova, L. G.
Netrusov, A. I.
author_sort Ding, F.
collection PubMed
description The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from different regions are lactic acid bacteria (LAB; lactobacilli, lactococci, and Leuconostoc spp. in different ratios) and different types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia.
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spelling pubmed-99400722023-02-21 Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing Ding, F. Krasilnikova, A. A. Leontieva, M. R. Stoyanova, L. G. Netrusov, A. I. Moscow Univ Biol Sci Bull Short Communications The taxonomic composition and spatial localization of yeast and bacteria in kefir grains (KG) obtained for study from different regions of the planet were investigated. The diversity of their microbiome has been demonstrated by high-throughput sequencing of bacterial 16S rRNA genes and the ITS1 region of the 18S-ITS1-5.8S-ITS2-28S complex of yeast rRNA. It has been established that the main representatives of the complex community of KG from different regions are lactic acid bacteria (LAB; lactobacilli, lactococci, and Leuconostoc spp. in different ratios) and different types of yeast of the genus Kazachstania (family Saccharomycetaceae). Acetic acid bacteria and a small percentage of yeast Kluyveromyces marxianus were detected in the KG from Tibet, and yeast Pichia kluyveri was detected in the KG from Ossetia. Pleiades Publishing 2023-02-20 2022 /pmc/articles/PMC9940072/ /pubmed/36843649 http://dx.doi.org/10.3103/S0096392522040010 Text en © Allerton Press, Inc. 2022, ISSN 0096-3925, Moscow University Biological Sciences Bulletin, 2022, Vol. 77, No. 4, pp. 286–291. © Allerton Press, Inc., 2022.Russian Text © The Author(s), 2022, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2022, Vol. 77, No. 4, pp. 265–272. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Short Communications
Ding, F.
Krasilnikova, A. A.
Leontieva, M. R.
Stoyanova, L. G.
Netrusov, A. I.
Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
title Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
title_full Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
title_fullStr Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
title_full_unstemmed Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
title_short Analysis of Kefir Grains from Different Regions of the Planet Using High-Throughput Sequencing
title_sort analysis of kefir grains from different regions of the planet using high-throughput sequencing
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940072/
https://www.ncbi.nlm.nih.gov/pubmed/36843649
http://dx.doi.org/10.3103/S0096392522040010
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