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Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis

INTRODUCTION: Cystitis glandularis (CG) is a rare chronic bladder hyperplastic disease that mainly manifests by recurrent frequent urination, dysuria and gross hematuria. The current lack of unified diagnosis and treatment criteria makes it essential to comprehensively describe the inflammatory immu...

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Autores principales: Zhou, Tai Lai, Chen, Heng Xin, Wang, Yin Zhao, Wen, Si Jie, Dao, Ping Hong, Wang, Yu Hang, Chen, Min Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940314/
https://www.ncbi.nlm.nih.gov/pubmed/36814917
http://dx.doi.org/10.3389/fimmu.2023.1083598
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author Zhou, Tai Lai
Chen, Heng Xin
Wang, Yin Zhao
Wen, Si Jie
Dao, Ping Hong
Wang, Yu Hang
Chen, Min Feng
author_facet Zhou, Tai Lai
Chen, Heng Xin
Wang, Yin Zhao
Wen, Si Jie
Dao, Ping Hong
Wang, Yu Hang
Chen, Min Feng
author_sort Zhou, Tai Lai
collection PubMed
description INTRODUCTION: Cystitis glandularis (CG) is a rare chronic bladder hyperplastic disease that mainly manifests by recurrent frequent urination, dysuria and gross hematuria. The current lack of unified diagnosis and treatment criteria makes it essential to comprehensively describe the inflammatory immune environment in CG research. METHODS: Here, we performed scRNA-sequencing in CG patients for the first time, in which four inflamed tissues as well as three surrounding normal bladder mucosa tissues were included. Specifically, we isolated 18,869 cells to conduct bioinformatic analysis and performed immunofluorescence experiments. RESULTS: Our genetic results demonstrate that CG does not have the classic chromosomal variation observed in bladder tumors, reveal the specific effects of TNF in KRT15 epithelial cells, and identify a new population of PIGR epithelial cells with high immunogenicity. In addition, we confirmed the activation difference of various kinds of T cells during chronic bladder inflammation and discovered a new group of CD27-Switch memory B cells expressing a variety of immunoglobulins. DISCUSSION: CG was regarded as a rare disease and its basic study is still weak.Our study reveals, for the first time, the different kinds of cell subgroups in CG and provides the necessary basis for the clinical treatment of cystitis glandularis. Besides, our study significantly advances the research on cystitis glandularis at the cellular level and provides a theoretical basis for the future treatment of cystitis glandularis.
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spelling pubmed-99403142023-02-21 Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis Zhou, Tai Lai Chen, Heng Xin Wang, Yin Zhao Wen, Si Jie Dao, Ping Hong Wang, Yu Hang Chen, Min Feng Front Immunol Immunology INTRODUCTION: Cystitis glandularis (CG) is a rare chronic bladder hyperplastic disease that mainly manifests by recurrent frequent urination, dysuria and gross hematuria. The current lack of unified diagnosis and treatment criteria makes it essential to comprehensively describe the inflammatory immune environment in CG research. METHODS: Here, we performed scRNA-sequencing in CG patients for the first time, in which four inflamed tissues as well as three surrounding normal bladder mucosa tissues were included. Specifically, we isolated 18,869 cells to conduct bioinformatic analysis and performed immunofluorescence experiments. RESULTS: Our genetic results demonstrate that CG does not have the classic chromosomal variation observed in bladder tumors, reveal the specific effects of TNF in KRT15 epithelial cells, and identify a new population of PIGR epithelial cells with high immunogenicity. In addition, we confirmed the activation difference of various kinds of T cells during chronic bladder inflammation and discovered a new group of CD27-Switch memory B cells expressing a variety of immunoglobulins. DISCUSSION: CG was regarded as a rare disease and its basic study is still weak.Our study reveals, for the first time, the different kinds of cell subgroups in CG and provides the necessary basis for the clinical treatment of cystitis glandularis. Besides, our study significantly advances the research on cystitis glandularis at the cellular level and provides a theoretical basis for the future treatment of cystitis glandularis. Frontiers Media S.A. 2023-02-06 /pmc/articles/PMC9940314/ /pubmed/36814917 http://dx.doi.org/10.3389/fimmu.2023.1083598 Text en Copyright © 2023 Zhou, Chen, Wang, Wen, Dao, Wang and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zhou, Tai Lai
Chen, Heng Xin
Wang, Yin Zhao
Wen, Si Jie
Dao, Ping Hong
Wang, Yu Hang
Chen, Min Feng
Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
title Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
title_full Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
title_fullStr Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
title_full_unstemmed Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
title_short Single-cell RNA sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
title_sort single-cell rna sequencing reveals the immune microenvironment and signaling networks in cystitis glandularis
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940314/
https://www.ncbi.nlm.nih.gov/pubmed/36814917
http://dx.doi.org/10.3389/fimmu.2023.1083598
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