Cargando…
Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis
Vascular plants reinforce the cell walls of the different xylem cell types with lignin phenolic polymers. Distinct lignin chemistries differ between each cell wall layer and each cell type to support their specific functions. Yet the mechanisms controlling the tight spatial localization of specific...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940878/ https://www.ncbi.nlm.nih.gov/pubmed/36449969 http://dx.doi.org/10.1093/plcell/koac344 |
_version_ | 1784891167617318912 |
---|---|
author | Blaschek, Leonard Murozuka, Emiko Serk, Henrik Ménard, Delphine Pesquet, Edouard |
author_facet | Blaschek, Leonard Murozuka, Emiko Serk, Henrik Ménard, Delphine Pesquet, Edouard |
author_sort | Blaschek, Leonard |
collection | PubMed |
description | Vascular plants reinforce the cell walls of the different xylem cell types with lignin phenolic polymers. Distinct lignin chemistries differ between each cell wall layer and each cell type to support their specific functions. Yet the mechanisms controlling the tight spatial localization of specific lignin chemistries remain unclear. Current hypotheses focus on control by monomer biosynthesis and/or export, while cell wall polymerization is viewed as random and nonlimiting. Here, we show that combinations of multiple individual laccases (LACs) are nonredundantly and specifically required to set the lignin chemistry in different cell types and their distinct cell wall layers. We dissected the roles of Arabidopsis thaliana LAC4, 5, 10, 12, and 17 by generating quadruple and quintuple loss-of-function mutants. Loss of these LACs in different combinations led to specific changes in lignin chemistry affecting both residue ring structures and/or aliphatic tails in specific cell types and cell wall layers. Moreover, we showed that LAC-mediated lignification has distinct functions in specific cell types, waterproofing fibers, and strengthening vessels. Altogether, we propose that the spatial control of lignin chemistry depends on different combinations of LACs with nonredundant activities immobilized in specific cell types and cell wall layers. |
format | Online Article Text |
id | pubmed-9940878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-99408782023-02-21 Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis Blaschek, Leonard Murozuka, Emiko Serk, Henrik Ménard, Delphine Pesquet, Edouard Plant Cell Research Article Vascular plants reinforce the cell walls of the different xylem cell types with lignin phenolic polymers. Distinct lignin chemistries differ between each cell wall layer and each cell type to support their specific functions. Yet the mechanisms controlling the tight spatial localization of specific lignin chemistries remain unclear. Current hypotheses focus on control by monomer biosynthesis and/or export, while cell wall polymerization is viewed as random and nonlimiting. Here, we show that combinations of multiple individual laccases (LACs) are nonredundantly and specifically required to set the lignin chemistry in different cell types and their distinct cell wall layers. We dissected the roles of Arabidopsis thaliana LAC4, 5, 10, 12, and 17 by generating quadruple and quintuple loss-of-function mutants. Loss of these LACs in different combinations led to specific changes in lignin chemistry affecting both residue ring structures and/or aliphatic tails in specific cell types and cell wall layers. Moreover, we showed that LAC-mediated lignification has distinct functions in specific cell types, waterproofing fibers, and strengthening vessels. Altogether, we propose that the spatial control of lignin chemistry depends on different combinations of LACs with nonredundant activities immobilized in specific cell types and cell wall layers. Oxford University Press 2022-11-30 /pmc/articles/PMC9940878/ /pubmed/36449969 http://dx.doi.org/10.1093/plcell/koac344 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Blaschek, Leonard Murozuka, Emiko Serk, Henrik Ménard, Delphine Pesquet, Edouard Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis |
title | Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis |
title_full | Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis |
title_fullStr | Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis |
title_full_unstemmed | Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis |
title_short | Different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in Arabidopsis |
title_sort | different combinations of laccase paralogs nonredundantly control the amount and composition of lignin in specific cell types and cell wall layers in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9940878/ https://www.ncbi.nlm.nih.gov/pubmed/36449969 http://dx.doi.org/10.1093/plcell/koac344 |
work_keys_str_mv | AT blaschekleonard differentcombinationsoflaccaseparalogsnonredundantlycontroltheamountandcompositionoflignininspecificcelltypesandcellwalllayersinarabidopsis AT murozukaemiko differentcombinationsoflaccaseparalogsnonredundantlycontroltheamountandcompositionoflignininspecificcelltypesandcellwalllayersinarabidopsis AT serkhenrik differentcombinationsoflaccaseparalogsnonredundantlycontroltheamountandcompositionoflignininspecificcelltypesandcellwalllayersinarabidopsis AT menarddelphine differentcombinationsoflaccaseparalogsnonredundantlycontroltheamountandcompositionoflignininspecificcelltypesandcellwalllayersinarabidopsis AT pesquetedouard differentcombinationsoflaccaseparalogsnonredundantlycontroltheamountandcompositionoflignininspecificcelltypesandcellwalllayersinarabidopsis |