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Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis
INTRODUCTION: Central nervous system (CNS) diseases, such as neurodegenerative disorders and brain diseases caused by acute injuries, are important, yet challenging to study due to disease lesion locations and other complexities. METHODS: Utilizing the powerful method of spatial transcriptome analys...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941151/ https://www.ncbi.nlm.nih.gov/pubmed/36825001 http://dx.doi.org/10.3389/fimmu.2023.1123652 |
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author | Zhang, Le Badai, Jiayidaer Wang, Guan Ru, Xufang Song, Wenkai You, Yujie He, Jiaojiao Huang, Suna Feng, Hua Chen, Runsheng Zhao, Yi Chen, Yujie |
author_facet | Zhang, Le Badai, Jiayidaer Wang, Guan Ru, Xufang Song, Wenkai You, Yujie He, Jiaojiao Huang, Suna Feng, Hua Chen, Runsheng Zhao, Yi Chen, Yujie |
author_sort | Zhang, Le |
collection | PubMed |
description | INTRODUCTION: Central nervous system (CNS) diseases, such as neurodegenerative disorders and brain diseases caused by acute injuries, are important, yet challenging to study due to disease lesion locations and other complexities. METHODS: Utilizing the powerful method of spatial transcriptome analysis together with novel algorithms we developed for the study, we report here for the first time a 3D trajectory map of gene expression changes in the brain following acute neural injury using a mouse model of intraventricular hemorrhage (IVH). IVH is a common and representative complication after various acute brain injuries with severe mortality and mobility implications. RESULTS: Our data identified three main 3D global pseudospace-time trajectory bundles that represent the main neural circuits from the lateral ventricle to the hippocampus and primary cortex affected by experimental IVH stimulation. Further analysis indicated a rapid response in the primary cortex, as well as a direct and integrated effect on the hippocampus after IVH stimulation. DISCUSSION: These results are informative for understanding the pathophysiological changes, including the spatial and temporal patterns of gene expression changes, in IVH patients after acute brain injury, strategizing more effective clinical management regimens, and developing novel bioinformatics strategies for the study of other CNS diseases. The algorithm strategies used in this study are searchable via a web service (www.combio-lezhang.online/3dstivh/home). |
format | Online Article Text |
id | pubmed-9941151 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99411512023-02-22 Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis Zhang, Le Badai, Jiayidaer Wang, Guan Ru, Xufang Song, Wenkai You, Yujie He, Jiaojiao Huang, Suna Feng, Hua Chen, Runsheng Zhao, Yi Chen, Yujie Front Immunol Immunology INTRODUCTION: Central nervous system (CNS) diseases, such as neurodegenerative disorders and brain diseases caused by acute injuries, are important, yet challenging to study due to disease lesion locations and other complexities. METHODS: Utilizing the powerful method of spatial transcriptome analysis together with novel algorithms we developed for the study, we report here for the first time a 3D trajectory map of gene expression changes in the brain following acute neural injury using a mouse model of intraventricular hemorrhage (IVH). IVH is a common and representative complication after various acute brain injuries with severe mortality and mobility implications. RESULTS: Our data identified three main 3D global pseudospace-time trajectory bundles that represent the main neural circuits from the lateral ventricle to the hippocampus and primary cortex affected by experimental IVH stimulation. Further analysis indicated a rapid response in the primary cortex, as well as a direct and integrated effect on the hippocampus after IVH stimulation. DISCUSSION: These results are informative for understanding the pathophysiological changes, including the spatial and temporal patterns of gene expression changes, in IVH patients after acute brain injury, strategizing more effective clinical management regimens, and developing novel bioinformatics strategies for the study of other CNS diseases. The algorithm strategies used in this study are searchable via a web service (www.combio-lezhang.online/3dstivh/home). Frontiers Media S.A. 2023-02-07 /pmc/articles/PMC9941151/ /pubmed/36825001 http://dx.doi.org/10.3389/fimmu.2023.1123652 Text en Copyright © 2023 Zhang, Badai, Wang, Ru, Song, You, He, Huang, Feng, Chen, Zhao and Chen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Zhang, Le Badai, Jiayidaer Wang, Guan Ru, Xufang Song, Wenkai You, Yujie He, Jiaojiao Huang, Suna Feng, Hua Chen, Runsheng Zhao, Yi Chen, Yujie Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
title | Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
title_full | Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
title_fullStr | Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
title_full_unstemmed | Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
title_short | Discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
title_sort | discovering hematoma-stimulated circuits for secondary brain injury after intraventricular hemorrhage by spatial transcriptome analysis |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941151/ https://www.ncbi.nlm.nih.gov/pubmed/36825001 http://dx.doi.org/10.3389/fimmu.2023.1123652 |
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