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Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity
Citrus HLB caused by Candidatus Liberibacter asiaticus is a pathogen-triggered immune disease. Here, we identified putative genetic determinants of HLB pathogenicity by integrating citrus genomic resources to characterize the pan-genome of accessions that differ in their response to HLB. Genome-wide...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941208/ https://www.ncbi.nlm.nih.gov/pubmed/36824272 http://dx.doi.org/10.1016/j.isci.2023.106024 |
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author | Gao, Yuxia Xu, Jin Li, Zhilong Zhang, Yunzeng Riera, Nadia Xiong, Zhiwei Ouyang, Zhigang Liu, Xinjun Lu, Zhanjun Seymour, Danelle Zhong, Balian Wang, Nian |
author_facet | Gao, Yuxia Xu, Jin Li, Zhilong Zhang, Yunzeng Riera, Nadia Xiong, Zhiwei Ouyang, Zhigang Liu, Xinjun Lu, Zhanjun Seymour, Danelle Zhong, Balian Wang, Nian |
author_sort | Gao, Yuxia |
collection | PubMed |
description | Citrus HLB caused by Candidatus Liberibacter asiaticus is a pathogen-triggered immune disease. Here, we identified putative genetic determinants of HLB pathogenicity by integrating citrus genomic resources to characterize the pan-genome of accessions that differ in their response to HLB. Genome-wide association mapping and analysis of allele-specific expression between susceptible, tolerant, and resistant accessions further refined candidates underlying the response to HLB. We first developed a phased diploid assembly of Citrus sinensis ‘Newhall’ genome and produced resequencing data for 91 citrus accessions that differ in their response to HLB. These data were combined with previous resequencing data from 356 accessions for genome-wide association mapping of the HLB response. Genes determinants for HLB pathogenicity were associated with host immune response, ROS production, and antioxidants. Overall, this study has provided a significant resource of citrus genomic data and identified candidate genes to be further explored to understand the genetic determinants of HLB pathogenicity. |
format | Online Article Text |
id | pubmed-9941208 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-99412082023-02-22 Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity Gao, Yuxia Xu, Jin Li, Zhilong Zhang, Yunzeng Riera, Nadia Xiong, Zhiwei Ouyang, Zhigang Liu, Xinjun Lu, Zhanjun Seymour, Danelle Zhong, Balian Wang, Nian iScience Article Citrus HLB caused by Candidatus Liberibacter asiaticus is a pathogen-triggered immune disease. Here, we identified putative genetic determinants of HLB pathogenicity by integrating citrus genomic resources to characterize the pan-genome of accessions that differ in their response to HLB. Genome-wide association mapping and analysis of allele-specific expression between susceptible, tolerant, and resistant accessions further refined candidates underlying the response to HLB. We first developed a phased diploid assembly of Citrus sinensis ‘Newhall’ genome and produced resequencing data for 91 citrus accessions that differ in their response to HLB. These data were combined with previous resequencing data from 356 accessions for genome-wide association mapping of the HLB response. Genes determinants for HLB pathogenicity were associated with host immune response, ROS production, and antioxidants. Overall, this study has provided a significant resource of citrus genomic data and identified candidate genes to be further explored to understand the genetic determinants of HLB pathogenicity. Elsevier 2023-01-23 /pmc/articles/PMC9941208/ /pubmed/36824272 http://dx.doi.org/10.1016/j.isci.2023.106024 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Gao, Yuxia Xu, Jin Li, Zhilong Zhang, Yunzeng Riera, Nadia Xiong, Zhiwei Ouyang, Zhigang Liu, Xinjun Lu, Zhanjun Seymour, Danelle Zhong, Balian Wang, Nian Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity |
title | Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity |
title_full | Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity |
title_fullStr | Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity |
title_full_unstemmed | Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity |
title_short | Citrus genomic resources unravel putative genetic determinants of Huanglongbing pathogenicity |
title_sort | citrus genomic resources unravel putative genetic determinants of huanglongbing pathogenicity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941208/ https://www.ncbi.nlm.nih.gov/pubmed/36824272 http://dx.doi.org/10.1016/j.isci.2023.106024 |
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