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Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat

The second most prevalent neurodegenerative ailment, Parkinson’s disease (PD), is characterized by both motor and non-motor symptoms. Levodopa is the backbone of treatment for PD at the moment. However, levodopa-induced side effects, such as dyskinesia, are commonly seen in PD patients. Recently, se...

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Autores principales: Sheng, Shuo, Li, Xianwei, Zhao, Shuo, Zheng, Changqing, Zhang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941341/
https://www.ncbi.nlm.nih.gov/pubmed/36824263
http://dx.doi.org/10.3389/fnagi.2023.1122712
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author Sheng, Shuo
Li, Xianwei
Zhao, Shuo
Zheng, Changqing
Zhang, Feng
author_facet Sheng, Shuo
Li, Xianwei
Zhao, Shuo
Zheng, Changqing
Zhang, Feng
author_sort Sheng, Shuo
collection PubMed
description The second most prevalent neurodegenerative ailment, Parkinson’s disease (PD), is characterized by both motor and non-motor symptoms. Levodopa is the backbone of treatment for PD at the moment. However, levodopa-induced side effects, such as dyskinesia, are commonly seen in PD patients. Recently, several antibiotics were found to present neuroprotective properties against neurodegenerative and neuro-inflammatory processes, which might be developed to effective therapies against PD. In this study, we aimed to identify if levodopa treatment could influence the gut bacterial antibiotic resistance in PD rat. Fecal samples were collected from healthy rats and 6-OHDA induced PD rats treated with different doses of levodopa, metagenomic sequencing data showed that levodopa resulted in gut bacteria composition change, the biomarkers of gut bacteria analyzed by LEfSe changed as well. More interestingly, compared with levodopa (5 mg/kg)-treated or no levodopa-treated PD rats, levodopa (10 mg/kg) caused a significant decrease in the abundance of tetW and vanTG genes in intestinal bacteria, which were related to tetracycline and vancomycin resistance, while the abundance of AAC6-lb-Suzhou gene increased apparently, which was related to aminoglycosides resistance, even though the total quantity of Antibiotic Resistance Gene (ARG) and Antibiotic Resistance Ontology (ARO) among all groups did not significantly differ. Consequently, our results imply that the combination of levodopa and antibiotics, such as tetracycline and vancomycin, in the treatment of PD may decrease the amount of corresponding antibiotic resistance genes in gut bacteria, which would give a theoretical basis for treating PD with levodopa combined with tetracycline and vancomycin in the future.
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spelling pubmed-99413412023-02-22 Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat Sheng, Shuo Li, Xianwei Zhao, Shuo Zheng, Changqing Zhang, Feng Front Aging Neurosci Aging Neuroscience The second most prevalent neurodegenerative ailment, Parkinson’s disease (PD), is characterized by both motor and non-motor symptoms. Levodopa is the backbone of treatment for PD at the moment. However, levodopa-induced side effects, such as dyskinesia, are commonly seen in PD patients. Recently, several antibiotics were found to present neuroprotective properties against neurodegenerative and neuro-inflammatory processes, which might be developed to effective therapies against PD. In this study, we aimed to identify if levodopa treatment could influence the gut bacterial antibiotic resistance in PD rat. Fecal samples were collected from healthy rats and 6-OHDA induced PD rats treated with different doses of levodopa, metagenomic sequencing data showed that levodopa resulted in gut bacteria composition change, the biomarkers of gut bacteria analyzed by LEfSe changed as well. More interestingly, compared with levodopa (5 mg/kg)-treated or no levodopa-treated PD rats, levodopa (10 mg/kg) caused a significant decrease in the abundance of tetW and vanTG genes in intestinal bacteria, which were related to tetracycline and vancomycin resistance, while the abundance of AAC6-lb-Suzhou gene increased apparently, which was related to aminoglycosides resistance, even though the total quantity of Antibiotic Resistance Gene (ARG) and Antibiotic Resistance Ontology (ARO) among all groups did not significantly differ. Consequently, our results imply that the combination of levodopa and antibiotics, such as tetracycline and vancomycin, in the treatment of PD may decrease the amount of corresponding antibiotic resistance genes in gut bacteria, which would give a theoretical basis for treating PD with levodopa combined with tetracycline and vancomycin in the future. Frontiers Media S.A. 2023-02-07 /pmc/articles/PMC9941341/ /pubmed/36824263 http://dx.doi.org/10.3389/fnagi.2023.1122712 Text en Copyright © 2023 Sheng, Li, Zhao, Zheng and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Aging Neuroscience
Sheng, Shuo
Li, Xianwei
Zhao, Shuo
Zheng, Changqing
Zhang, Feng
Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat
title Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat
title_full Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat
title_fullStr Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat
title_full_unstemmed Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat
title_short Effects of levodopa on gut bacterial antibiotic resistance in Parkinson’s disease rat
title_sort effects of levodopa on gut bacterial antibiotic resistance in parkinson’s disease rat
topic Aging Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941341/
https://www.ncbi.nlm.nih.gov/pubmed/36824263
http://dx.doi.org/10.3389/fnagi.2023.1122712
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