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Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration
The brain-bone regulatory system regulates skeletal homeostasis via bioactive neuropeptides, yet the underlying mechanism remains elusive. Here, we report the role of the neuropeptide VF (NPVF, VPNLPQRF-NH(2)) in enhancing both angiogenesis and osteogenesis in a rat skeletal system and the potential...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941492/ https://www.ncbi.nlm.nih.gov/pubmed/36804475 http://dx.doi.org/10.1038/s42003-023-04567-x |
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author | Yu, Hongping Wang, Yanyi Gao, Junjie Gao, Youshui Zhong, Chao Chen, Yixuan |
author_facet | Yu, Hongping Wang, Yanyi Gao, Junjie Gao, Youshui Zhong, Chao Chen, Yixuan |
author_sort | Yu, Hongping |
collection | PubMed |
description | The brain-bone regulatory system regulates skeletal homeostasis via bioactive neuropeptides, yet the underlying mechanism remains elusive. Here, we report the role of the neuropeptide VF (NPVF, VPNLPQRF-NH(2)) in enhancing both angiogenesis and osteogenesis in a rat skeletal system and the potential pathways involved. An in vitro study revealed that NPVF not only promotes migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) by activating NPFFR1, which leads to upregulation of miR-181c-3p and downregulation of Argonaute1 (AGO1), but also mediates osteogenic differentiation of bone mesenchymal stem cells (BMSCs) via the Wnt/β-catenin signaling pathway. To improve the stability and bioavailability and thus efficacy of NPVF as a promoter of in vivo bone regeneration, we genetically engineered amyloid-NPVF-fusion proteins and utilized them as self-assembling nanofiber coatings to treat bone defects in a rat calvarial defect model. We found that a porous hydroxyapatite scaffold loaded with the NPVF peptide-fused amyloid coating substantially enhanced angiogenesis and site-specific fresh bone in-growth when implanted in calvarial defects. Taken together, our work uncovered a previously undefined crosstalk between the brain and bone by unveiling the role of NPVF in bone tissue and demonstrated a viable method for promoting bone tissue repairs based upon self-assembling NPVF-containing protein coatings. |
format | Online Article Text |
id | pubmed-9941492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99414922023-02-22 Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration Yu, Hongping Wang, Yanyi Gao, Junjie Gao, Youshui Zhong, Chao Chen, Yixuan Commun Biol Article The brain-bone regulatory system regulates skeletal homeostasis via bioactive neuropeptides, yet the underlying mechanism remains elusive. Here, we report the role of the neuropeptide VF (NPVF, VPNLPQRF-NH(2)) in enhancing both angiogenesis and osteogenesis in a rat skeletal system and the potential pathways involved. An in vitro study revealed that NPVF not only promotes migration and angiogenesis of human umbilical vein endothelial cells (HUVECs) by activating NPFFR1, which leads to upregulation of miR-181c-3p and downregulation of Argonaute1 (AGO1), but also mediates osteogenic differentiation of bone mesenchymal stem cells (BMSCs) via the Wnt/β-catenin signaling pathway. To improve the stability and bioavailability and thus efficacy of NPVF as a promoter of in vivo bone regeneration, we genetically engineered amyloid-NPVF-fusion proteins and utilized them as self-assembling nanofiber coatings to treat bone defects in a rat calvarial defect model. We found that a porous hydroxyapatite scaffold loaded with the NPVF peptide-fused amyloid coating substantially enhanced angiogenesis and site-specific fresh bone in-growth when implanted in calvarial defects. Taken together, our work uncovered a previously undefined crosstalk between the brain and bone by unveiling the role of NPVF in bone tissue and demonstrated a viable method for promoting bone tissue repairs based upon self-assembling NPVF-containing protein coatings. Nature Publishing Group UK 2023-02-20 /pmc/articles/PMC9941492/ /pubmed/36804475 http://dx.doi.org/10.1038/s42003-023-04567-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yu, Hongping Wang, Yanyi Gao, Junjie Gao, Youshui Zhong, Chao Chen, Yixuan Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration |
title | Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration |
title_full | Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration |
title_fullStr | Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration |
title_full_unstemmed | Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration |
title_short | Application of the neuropeptide NPVF to enhance angiogenesis and osteogenesis in bone regeneration |
title_sort | application of the neuropeptide npvf to enhance angiogenesis and osteogenesis in bone regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941492/ https://www.ncbi.nlm.nih.gov/pubmed/36804475 http://dx.doi.org/10.1038/s42003-023-04567-x |
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