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Pixel-by-pixel autofluorescence corrected FRET in fluorescence microscopy improves accuracy for samples with spatially varied autofluorescence to signal ratio

The actual interaction between signaling species in cellular processes is often more important than their expression levels. Förster resonance energy transfer (FRET) is a popular tool for studying molecular interactions, since it is highly sensitive to proximity in the range of 2–10 nm. Spectral spi...

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Detalles Bibliográficos
Autores principales: Rebenku, István, Lloyd, Cameron B., Szöllősi, János, Vereb, György
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941493/
https://www.ncbi.nlm.nih.gov/pubmed/36804608
http://dx.doi.org/10.1038/s41598-023-30098-w