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Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment
Cadmium, one of the toxic heavy metals, robustly impact crop growth and development and food safety. In this study, the mechanisms of wheat (Triticum aestivum L.) selenium-binding protein-A (TaSBP-A) involved in response to Cd stress was fully investigated by overexpression in Arabidopsis and wheat....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941557/ https://www.ncbi.nlm.nih.gov/pubmed/36824198 http://dx.doi.org/10.3389/fpls.2023.1103241 |
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author | Luo, Fei Zhu, Dong Sun, Haocheng Zou, Rong Duan, Wenjing Liu, Junxian Yan, Yueming |
author_facet | Luo, Fei Zhu, Dong Sun, Haocheng Zou, Rong Duan, Wenjing Liu, Junxian Yan, Yueming |
author_sort | Luo, Fei |
collection | PubMed |
description | Cadmium, one of the toxic heavy metals, robustly impact crop growth and development and food safety. In this study, the mechanisms of wheat (Triticum aestivum L.) selenium-binding protein-A (TaSBP-A) involved in response to Cd stress was fully investigated by overexpression in Arabidopsis and wheat. As a cytoplasm protein, TaSBP-A showed a high expression in plant roots and its expression levels were highly induced by Cd treatment. The overexpression of TaSBP-A enhanced Cd-toleration in yeast, Arabidopsis and wheat. Meanwhile, transgenic Arabidopsis under Cd stress showed a lower H(2)O(2) and malondialdehyde content and a higher photochemical efficiency in the leaf and a reduction of free Cd(2+) in the root. Transgenic wheat seedlings of TaSBP exhibited an increment of Cd content in the root, and a reduction Cd content in the leaf under Cd(2+) stress. Cd(2+) binding assay combined with a thermodynamics survey and secondary structure analysis indicated that the unique CXXC motif in TaSBP was a major Cd-binding site participating in the Cd detoxification. These results suggested that TaSBP-A can enhance the sequestration of free Cd(2+) in root and inhibit the Cd transfer from root to leaf, ultimately conferring plant Cd-tolerance via alleviating the oxidative stress and photosynthesis impairment triggered by Cd stress. |
format | Online Article Text |
id | pubmed-9941557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99415572023-02-22 Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment Luo, Fei Zhu, Dong Sun, Haocheng Zou, Rong Duan, Wenjing Liu, Junxian Yan, Yueming Front Plant Sci Plant Science Cadmium, one of the toxic heavy metals, robustly impact crop growth and development and food safety. In this study, the mechanisms of wheat (Triticum aestivum L.) selenium-binding protein-A (TaSBP-A) involved in response to Cd stress was fully investigated by overexpression in Arabidopsis and wheat. As a cytoplasm protein, TaSBP-A showed a high expression in plant roots and its expression levels were highly induced by Cd treatment. The overexpression of TaSBP-A enhanced Cd-toleration in yeast, Arabidopsis and wheat. Meanwhile, transgenic Arabidopsis under Cd stress showed a lower H(2)O(2) and malondialdehyde content and a higher photochemical efficiency in the leaf and a reduction of free Cd(2+) in the root. Transgenic wheat seedlings of TaSBP exhibited an increment of Cd content in the root, and a reduction Cd content in the leaf under Cd(2+) stress. Cd(2+) binding assay combined with a thermodynamics survey and secondary structure analysis indicated that the unique CXXC motif in TaSBP was a major Cd-binding site participating in the Cd detoxification. These results suggested that TaSBP-A can enhance the sequestration of free Cd(2+) in root and inhibit the Cd transfer from root to leaf, ultimately conferring plant Cd-tolerance via alleviating the oxidative stress and photosynthesis impairment triggered by Cd stress. Frontiers Media S.A. 2023-02-07 /pmc/articles/PMC9941557/ /pubmed/36824198 http://dx.doi.org/10.3389/fpls.2023.1103241 Text en Copyright © 2023 Luo, Zhu, Sun, Zou, Duan, Liu and Yan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Luo, Fei Zhu, Dong Sun, Haocheng Zou, Rong Duan, Wenjing Liu, Junxian Yan, Yueming Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
title | Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
title_full | Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
title_fullStr | Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
title_full_unstemmed | Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
title_short | Wheat Selenium-binding protein TaSBP-A enhances cadmium tolerance by decreasing free Cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
title_sort | wheat selenium-binding protein tasbp-a enhances cadmium tolerance by decreasing free cd(2+) and alleviating the oxidative damage and photosynthesis impairment |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941557/ https://www.ncbi.nlm.nih.gov/pubmed/36824198 http://dx.doi.org/10.3389/fpls.2023.1103241 |
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