A simple and accessible CRISPR genome editing laboratory exercise using yeast

CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in...

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Detalles Bibliográficos
Autores principales: Shortt, Connor, Krippaehne, Elise, Wasko, Brian M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941855/
https://www.ncbi.nlm.nih.gov/pubmed/36824382
http://dx.doi.org/10.17912/micropub.biology.000699
Descripción
Sumario:CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in a single laboratory session and to be accessible to students and instructors without experience working with yeast.

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