A simple and accessible CRISPR genome editing laboratory exercise using yeast

CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in...

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Detalles Bibliográficos
Autores principales: Shortt, Connor, Krippaehne, Elise, Wasko, Brian M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941855/
https://www.ncbi.nlm.nih.gov/pubmed/36824382
http://dx.doi.org/10.17912/micropub.biology.000699
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author Shortt, Connor
Krippaehne, Elise
Wasko, Brian M
author_facet Shortt, Connor
Krippaehne, Elise
Wasko, Brian M
author_sort Shortt, Connor
collection PubMed
description CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in a single laboratory session and to be accessible to students and instructors without experience working with yeast.
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spelling pubmed-99418552023-02-22 A simple and accessible CRISPR genome editing laboratory exercise using yeast Shortt, Connor Krippaehne, Elise Wasko, Brian M MicroPubl Biol New Methods CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in a single laboratory session and to be accessible to students and instructors without experience working with yeast. Caltech Library 2023-02-06 /pmc/articles/PMC9941855/ /pubmed/36824382 http://dx.doi.org/10.17912/micropub.biology.000699 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Shortt, Connor
Krippaehne, Elise
Wasko, Brian M
A simple and accessible CRISPR genome editing laboratory exercise using yeast
title A simple and accessible CRISPR genome editing laboratory exercise using yeast
title_full A simple and accessible CRISPR genome editing laboratory exercise using yeast
title_fullStr A simple and accessible CRISPR genome editing laboratory exercise using yeast
title_full_unstemmed A simple and accessible CRISPR genome editing laboratory exercise using yeast
title_short A simple and accessible CRISPR genome editing laboratory exercise using yeast
title_sort simple and accessible crispr genome editing laboratory exercise using yeast
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9941855/
https://www.ncbi.nlm.nih.gov/pubmed/36824382
http://dx.doi.org/10.17912/micropub.biology.000699
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