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Miniature CRISPR-Cas12f1-Mediated Single-Nucleotide Microbial Genome Editing Using 3′-Truncated sgRNA

The CRISPR-Cas system has been used as a convenient tool for genome editing because the nuclease that cuts the target DNA and the guide RNA that recognizes the target are separated into modules. Cas12f1, which has a smaller size than that of other Cas nucleases, is easily loaded into vectors and is...

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Detalles Bibliográficos
Autores principales:	Lee, Ho Joung, Kim, Hyun Ju, Lee, Sang Jun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc., publishers 2023
Materias:	Original Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942177/ https://www.ncbi.nlm.nih.gov/pubmed/36576897 http://dx.doi.org/10.1089/crispr.2022.0071

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