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Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid

[Image: see text] Gallic acid is a prevalent secondary plant metabolite distinguished as one of the most effective free-radical scavengers among phenolic acids. This compound is also known for its cytotoxic, anti-inflammatory, and antimicrobial activities. Bulk quantities of gallic acid are conventi...

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Autores principales: Kutraite, Ingrida, Malys, Naglis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942251/
https://www.ncbi.nlm.nih.gov/pubmed/36724292
http://dx.doi.org/10.1021/acssynbio.2c00537
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author Kutraite, Ingrida
Malys, Naglis
author_facet Kutraite, Ingrida
Malys, Naglis
author_sort Kutraite, Ingrida
collection PubMed
description [Image: see text] Gallic acid is a prevalent secondary plant metabolite distinguished as one of the most effective free-radical scavengers among phenolic acids. This compound is also known for its cytotoxic, anti-inflammatory, and antimicrobial activities. Bulk quantities of gallic acid are conventionally produced by acid hydrolysis of tannins, a costly and environmentally hazardous process. With the aim to develop more sustainable approaches, microbial bioproduction strategies have been attempted recently. To advance synthetic biology and metabolic engineering of microorganisms for gallic acid production, we characterize here a transcription factor-based inducible system PpGalR/P(PP_RS13150) that responds to the extracellular gallic acid in a dose-dependent manner in Pseudomonas putida KT2440. Surprisingly, this compound does not mediate induction when PpGalR/P(PP_RS13150) is used in non-native host background. We show that the activation of the inducible system requires gallate dioxygenase activity encoded by galA gene. The 4-oxalomesaconic acid, an intermediate of gallic acid-metabolism, is identified as the effector molecule that interacts with the transcription factor GalR mediating activation of gene expression. Introduction of galA gene along galR enables development of biosensors suitable for detection and monitoring of gallic acid extracellularly using non-native hosts such as E. coli and C. necator. Moreover, the P. putida-based biosensor’s applicability is demonstrated by detecting and measuring gallic acid in extracts of Camellia sinensis leaves. This study reports the strategy, which can be applied for developing gallic acid biosensors using bacterial species outside Pseudomonas genus.
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spelling pubmed-99422512023-02-22 Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid Kutraite, Ingrida Malys, Naglis ACS Synth Biol [Image: see text] Gallic acid is a prevalent secondary plant metabolite distinguished as one of the most effective free-radical scavengers among phenolic acids. This compound is also known for its cytotoxic, anti-inflammatory, and antimicrobial activities. Bulk quantities of gallic acid are conventionally produced by acid hydrolysis of tannins, a costly and environmentally hazardous process. With the aim to develop more sustainable approaches, microbial bioproduction strategies have been attempted recently. To advance synthetic biology and metabolic engineering of microorganisms for gallic acid production, we characterize here a transcription factor-based inducible system PpGalR/P(PP_RS13150) that responds to the extracellular gallic acid in a dose-dependent manner in Pseudomonas putida KT2440. Surprisingly, this compound does not mediate induction when PpGalR/P(PP_RS13150) is used in non-native host background. We show that the activation of the inducible system requires gallate dioxygenase activity encoded by galA gene. The 4-oxalomesaconic acid, an intermediate of gallic acid-metabolism, is identified as the effector molecule that interacts with the transcription factor GalR mediating activation of gene expression. Introduction of galA gene along galR enables development of biosensors suitable for detection and monitoring of gallic acid extracellularly using non-native hosts such as E. coli and C. necator. Moreover, the P. putida-based biosensor’s applicability is demonstrated by detecting and measuring gallic acid in extracts of Camellia sinensis leaves. This study reports the strategy, which can be applied for developing gallic acid biosensors using bacterial species outside Pseudomonas genus. American Chemical Society 2023-02-01 /pmc/articles/PMC9942251/ /pubmed/36724292 http://dx.doi.org/10.1021/acssynbio.2c00537 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Kutraite, Ingrida
Malys, Naglis
Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid
title Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid
title_full Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid
title_fullStr Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid
title_full_unstemmed Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid
title_short Development and Application of Whole-Cell Biosensors for the Detection of Gallic Acid
title_sort development and application of whole-cell biosensors for the detection of gallic acid
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942251/
https://www.ncbi.nlm.nih.gov/pubmed/36724292
http://dx.doi.org/10.1021/acssynbio.2c00537
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