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Matrix metalloproteinase profiling and their roles in disease
Matrix metalloproteinases (MMPs) play roles in remodelling of the extracellular matrix that occurs during morphogenesis, repair, and angiogenesis. Dysregulation of extracellular matrix remodelling can lead to cell proliferation, invasion, and tissue fibrosis. Identification of a specific MMP(s) in a...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Royal Society of Chemistry
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942564/ https://www.ncbi.nlm.nih.gov/pubmed/36825288 http://dx.doi.org/10.1039/d2ra07005g |
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author | Chang, Mayland |
author_facet | Chang, Mayland |
author_sort | Chang, Mayland |
collection | PubMed |
description | Matrix metalloproteinases (MMPs) play roles in remodelling of the extracellular matrix that occurs during morphogenesis, repair, and angiogenesis. Dysregulation of extracellular matrix remodelling can lead to cell proliferation, invasion, and tissue fibrosis. Identification of a specific MMP(s) in a disease has been challenging due to the presence of 24 closely-related human MMPs, each existing in three forms, of which only one is active and capable of catalysis. This review focuses on methods for MMP profiling, with particular emphasis on the batimastat affinity resin that binds only to the active forms of MMPs and related ADAMs (a disintegrin and metalloproteinases), which are then identified by mass spectrometry. Use of the batimastat affinity resin has identified targets for intervention in several human diseases. |
format | Online Article Text |
id | pubmed-9942564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-99425642023-02-22 Matrix metalloproteinase profiling and their roles in disease Chang, Mayland RSC Adv Chemistry Matrix metalloproteinases (MMPs) play roles in remodelling of the extracellular matrix that occurs during morphogenesis, repair, and angiogenesis. Dysregulation of extracellular matrix remodelling can lead to cell proliferation, invasion, and tissue fibrosis. Identification of a specific MMP(s) in a disease has been challenging due to the presence of 24 closely-related human MMPs, each existing in three forms, of which only one is active and capable of catalysis. This review focuses on methods for MMP profiling, with particular emphasis on the batimastat affinity resin that binds only to the active forms of MMPs and related ADAMs (a disintegrin and metalloproteinases), which are then identified by mass spectrometry. Use of the batimastat affinity resin has identified targets for intervention in several human diseases. The Royal Society of Chemistry 2023-02-21 /pmc/articles/PMC9942564/ /pubmed/36825288 http://dx.doi.org/10.1039/d2ra07005g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Chang, Mayland Matrix metalloproteinase profiling and their roles in disease |
title | Matrix metalloproteinase profiling and their roles in disease |
title_full | Matrix metalloproteinase profiling and their roles in disease |
title_fullStr | Matrix metalloproteinase profiling and their roles in disease |
title_full_unstemmed | Matrix metalloproteinase profiling and their roles in disease |
title_short | Matrix metalloproteinase profiling and their roles in disease |
title_sort | matrix metalloproteinase profiling and their roles in disease |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942564/ https://www.ncbi.nlm.nih.gov/pubmed/36825288 http://dx.doi.org/10.1039/d2ra07005g |
work_keys_str_mv | AT changmayland matrixmetalloproteinaseprofilingandtheirrolesindisease |