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The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen

Entomopathogenic fungi infect insects by penetrating through the cuticle into the host body. To breach the host cuticle, some fungal pathogens produce specialized infection cells called appressoria, which develop enormous turgor pressure to allow cuticle penetration. However, regulatory mechanisms u...

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Autores principales: Wang, Lili, Lai, Yiling, Chen, Jingjing, Cao, Xuan, Zheng, Weilu, Dong, Ling, Zheng, Yitong, Li, Fang, Wei, Gang, Wang, Sibao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942893/
https://www.ncbi.nlm.nih.gov/pubmed/36649415
http://dx.doi.org/10.1073/pnas.2217145120
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author Wang, Lili
Lai, Yiling
Chen, Jingjing
Cao, Xuan
Zheng, Weilu
Dong, Ling
Zheng, Yitong
Li, Fang
Wei, Gang
Wang, Sibao
author_facet Wang, Lili
Lai, Yiling
Chen, Jingjing
Cao, Xuan
Zheng, Weilu
Dong, Ling
Zheng, Yitong
Li, Fang
Wei, Gang
Wang, Sibao
author_sort Wang, Lili
collection PubMed
description Entomopathogenic fungi infect insects by penetrating through the cuticle into the host body. To breach the host cuticle, some fungal pathogens produce specialized infection cells called appressoria, which develop enormous turgor pressure to allow cuticle penetration. However, regulatory mechanisms underlying appressorium turgor generation are poorly understood. Here, we show that the histone lysine methyltransferase ASH1 in the insecticidal fungus Metarhizium robertsii, which is strongly induced during infection of the mosquito cuticle, regulates appressorium turgor generation and cuticle penetration by activating the peroxin gene Mrpex16 via H3K36 dimethylation. MrPEX16 is required for the biogenesis of peroxisomes that participate in lipid catabolism and further promotes the hydrolysis of triacylglycerols stored in lipid droplets to produce glycerol for turgor generation, facilitating appressorium-mediated insect infection. Together, the ASH1–PEX16 pathway plays a pivotal role in regulating peroxisome biogenesis to promote lipolysis for appressorium turgor generation, providing insights into the molecular mechanisms underlying fungal pathogenesis.
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spelling pubmed-99428932023-07-17 The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen Wang, Lili Lai, Yiling Chen, Jingjing Cao, Xuan Zheng, Weilu Dong, Ling Zheng, Yitong Li, Fang Wei, Gang Wang, Sibao Proc Natl Acad Sci U S A Biological Sciences Entomopathogenic fungi infect insects by penetrating through the cuticle into the host body. To breach the host cuticle, some fungal pathogens produce specialized infection cells called appressoria, which develop enormous turgor pressure to allow cuticle penetration. However, regulatory mechanisms underlying appressorium turgor generation are poorly understood. Here, we show that the histone lysine methyltransferase ASH1 in the insecticidal fungus Metarhizium robertsii, which is strongly induced during infection of the mosquito cuticle, regulates appressorium turgor generation and cuticle penetration by activating the peroxin gene Mrpex16 via H3K36 dimethylation. MrPEX16 is required for the biogenesis of peroxisomes that participate in lipid catabolism and further promotes the hydrolysis of triacylglycerols stored in lipid droplets to produce glycerol for turgor generation, facilitating appressorium-mediated insect infection. Together, the ASH1–PEX16 pathway plays a pivotal role in regulating peroxisome biogenesis to promote lipolysis for appressorium turgor generation, providing insights into the molecular mechanisms underlying fungal pathogenesis. National Academy of Sciences 2023-01-17 2023-01-24 /pmc/articles/PMC9942893/ /pubmed/36649415 http://dx.doi.org/10.1073/pnas.2217145120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Wang, Lili
Lai, Yiling
Chen, Jingjing
Cao, Xuan
Zheng, Weilu
Dong, Ling
Zheng, Yitong
Li, Fang
Wei, Gang
Wang, Sibao
The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
title The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
title_full The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
title_fullStr The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
title_full_unstemmed The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
title_short The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
title_sort ash1–pex16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942893/
https://www.ncbi.nlm.nih.gov/pubmed/36649415
http://dx.doi.org/10.1073/pnas.2217145120
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