Cargando…
The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen
Entomopathogenic fungi infect insects by penetrating through the cuticle into the host body. To breach the host cuticle, some fungal pathogens produce specialized infection cells called appressoria, which develop enormous turgor pressure to allow cuticle penetration. However, regulatory mechanisms u...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942893/ https://www.ncbi.nlm.nih.gov/pubmed/36649415 http://dx.doi.org/10.1073/pnas.2217145120 |
_version_ | 1784891596209127424 |
---|---|
author | Wang, Lili Lai, Yiling Chen, Jingjing Cao, Xuan Zheng, Weilu Dong, Ling Zheng, Yitong Li, Fang Wei, Gang Wang, Sibao |
author_facet | Wang, Lili Lai, Yiling Chen, Jingjing Cao, Xuan Zheng, Weilu Dong, Ling Zheng, Yitong Li, Fang Wei, Gang Wang, Sibao |
author_sort | Wang, Lili |
collection | PubMed |
description | Entomopathogenic fungi infect insects by penetrating through the cuticle into the host body. To breach the host cuticle, some fungal pathogens produce specialized infection cells called appressoria, which develop enormous turgor pressure to allow cuticle penetration. However, regulatory mechanisms underlying appressorium turgor generation are poorly understood. Here, we show that the histone lysine methyltransferase ASH1 in the insecticidal fungus Metarhizium robertsii, which is strongly induced during infection of the mosquito cuticle, regulates appressorium turgor generation and cuticle penetration by activating the peroxin gene Mrpex16 via H3K36 dimethylation. MrPEX16 is required for the biogenesis of peroxisomes that participate in lipid catabolism and further promotes the hydrolysis of triacylglycerols stored in lipid droplets to produce glycerol for turgor generation, facilitating appressorium-mediated insect infection. Together, the ASH1–PEX16 pathway plays a pivotal role in regulating peroxisome biogenesis to promote lipolysis for appressorium turgor generation, providing insights into the molecular mechanisms underlying fungal pathogenesis. |
format | Online Article Text |
id | pubmed-9942893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-99428932023-07-17 The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen Wang, Lili Lai, Yiling Chen, Jingjing Cao, Xuan Zheng, Weilu Dong, Ling Zheng, Yitong Li, Fang Wei, Gang Wang, Sibao Proc Natl Acad Sci U S A Biological Sciences Entomopathogenic fungi infect insects by penetrating through the cuticle into the host body. To breach the host cuticle, some fungal pathogens produce specialized infection cells called appressoria, which develop enormous turgor pressure to allow cuticle penetration. However, regulatory mechanisms underlying appressorium turgor generation are poorly understood. Here, we show that the histone lysine methyltransferase ASH1 in the insecticidal fungus Metarhizium robertsii, which is strongly induced during infection of the mosquito cuticle, regulates appressorium turgor generation and cuticle penetration by activating the peroxin gene Mrpex16 via H3K36 dimethylation. MrPEX16 is required for the biogenesis of peroxisomes that participate in lipid catabolism and further promotes the hydrolysis of triacylglycerols stored in lipid droplets to produce glycerol for turgor generation, facilitating appressorium-mediated insect infection. Together, the ASH1–PEX16 pathway plays a pivotal role in regulating peroxisome biogenesis to promote lipolysis for appressorium turgor generation, providing insights into the molecular mechanisms underlying fungal pathogenesis. National Academy of Sciences 2023-01-17 2023-01-24 /pmc/articles/PMC9942893/ /pubmed/36649415 http://dx.doi.org/10.1073/pnas.2217145120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Wang, Lili Lai, Yiling Chen, Jingjing Cao, Xuan Zheng, Weilu Dong, Ling Zheng, Yitong Li, Fang Wei, Gang Wang, Sibao The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
title | The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
title_full | The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
title_fullStr | The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
title_full_unstemmed | The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
title_short | The ASH1–PEX16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
title_sort | ash1–pex16 regulatory pathway controls peroxisome biogenesis for appressorium-mediated insect infection by a fungal pathogen |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9942893/ https://www.ncbi.nlm.nih.gov/pubmed/36649415 http://dx.doi.org/10.1073/pnas.2217145120 |
work_keys_str_mv | AT wanglili theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT laiyiling theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT chenjingjing theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT caoxuan theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT zhengweilu theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT dongling theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT zhengyitong theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT lifang theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT weigang theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT wangsibao theash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT wanglili ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT laiyiling ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT chenjingjing ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT caoxuan ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT zhengweilu ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT dongling ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT zhengyitong ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT lifang ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT weigang ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen AT wangsibao ash1pex16regulatorypathwaycontrolsperoxisomebiogenesisforappressoriummediatedinsectinfectionbyafungalpathogen |