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Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study

The majority of Hepatitis E Virus (HEV)-related studies are carried out in adults whereas information about HEV seroprevalence, clinical disease manifestation, molecular epidemiology, and transmission patterns in children is limited. To estimate HEV seroprevalence among scholar children living in an...

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Autores principales: Fernández Villalobos, Nathalie Verónica, Kessel, Barbora, Torres Páez, Johanna Carolina, Strömpl, Julia, Kerrinnes, Tobias, de la Hoz Restrepo, Fernando Pio, Strengert, Monika, Krause, Gérard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943700/
https://www.ncbi.nlm.nih.gov/pubmed/36844812
http://dx.doi.org/10.3389/fpubh.2023.981172
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author Fernández Villalobos, Nathalie Verónica
Kessel, Barbora
Torres Páez, Johanna Carolina
Strömpl, Julia
Kerrinnes, Tobias
de la Hoz Restrepo, Fernando Pio
Strengert, Monika
Krause, Gérard
author_facet Fernández Villalobos, Nathalie Verónica
Kessel, Barbora
Torres Páez, Johanna Carolina
Strömpl, Julia
Kerrinnes, Tobias
de la Hoz Restrepo, Fernando Pio
Strengert, Monika
Krause, Gérard
author_sort Fernández Villalobos, Nathalie Verónica
collection PubMed
description The majority of Hepatitis E Virus (HEV)-related studies are carried out in adults whereas information about HEV seroprevalence, clinical disease manifestation, molecular epidemiology, and transmission patterns in children is limited. To estimate HEV seroprevalence among scholar children living in an urban setting and to analyze risk factors for an infection, we invited children aged 5–18 years from Bogotá (Colombia) for a cross-sectional survey. We collected self-reported data on demographics, social, clinical, and exposure variables in a structured interview. Venous blood samples were analyzed with two commercially available ELISAs for HEV-specific IgG antibodies. Among the 263 participants, we found three HEV IgG-reactive samples (1.1%) using both assays. We additionally characterized the samples for HEV IgM using a commercially available IgM ELISA and for HEV RNA. Here, we found one IgM-reactive sample, which was also reactive for IgG. In contrast, none of the IgM- and IgG-reactive sera samples showed detectable RNA levels indicating HEV exposure had not been recently. All participants reported access to drinking water and sanitary systems in their households and frequent hand washing routines (76–88%). Eighty percent of children reported no direct contact with pigs, but occasional pork consumption was common (90%). In contrast to the majority of studies performed in Colombian adults, we found a low unadjusted HEV seroprevalence of 1.1% (95% CI: 0.3–3.6%) for both HEV IgG ELISAs in our study population. While the majority of participants reported pork consumption, we speculate in the absence of viral RNA for genotyping in the affected individuals, that existing access to drinking water and sanitary systems within our study group contribute to the low HEV seroprevalence.
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spelling pubmed-99437002023-02-23 Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study Fernández Villalobos, Nathalie Verónica Kessel, Barbora Torres Páez, Johanna Carolina Strömpl, Julia Kerrinnes, Tobias de la Hoz Restrepo, Fernando Pio Strengert, Monika Krause, Gérard Front Public Health Public Health The majority of Hepatitis E Virus (HEV)-related studies are carried out in adults whereas information about HEV seroprevalence, clinical disease manifestation, molecular epidemiology, and transmission patterns in children is limited. To estimate HEV seroprevalence among scholar children living in an urban setting and to analyze risk factors for an infection, we invited children aged 5–18 years from Bogotá (Colombia) for a cross-sectional survey. We collected self-reported data on demographics, social, clinical, and exposure variables in a structured interview. Venous blood samples were analyzed with two commercially available ELISAs for HEV-specific IgG antibodies. Among the 263 participants, we found three HEV IgG-reactive samples (1.1%) using both assays. We additionally characterized the samples for HEV IgM using a commercially available IgM ELISA and for HEV RNA. Here, we found one IgM-reactive sample, which was also reactive for IgG. In contrast, none of the IgM- and IgG-reactive sera samples showed detectable RNA levels indicating HEV exposure had not been recently. All participants reported access to drinking water and sanitary systems in their households and frequent hand washing routines (76–88%). Eighty percent of children reported no direct contact with pigs, but occasional pork consumption was common (90%). In contrast to the majority of studies performed in Colombian adults, we found a low unadjusted HEV seroprevalence of 1.1% (95% CI: 0.3–3.6%) for both HEV IgG ELISAs in our study population. While the majority of participants reported pork consumption, we speculate in the absence of viral RNA for genotyping in the affected individuals, that existing access to drinking water and sanitary systems within our study group contribute to the low HEV seroprevalence. Frontiers Media S.A. 2023-02-07 /pmc/articles/PMC9943700/ /pubmed/36844812 http://dx.doi.org/10.3389/fpubh.2023.981172 Text en Copyright © 2023 Fernández Villalobos, Kessel, Torres Páez, Strömpl, Kerrinnes, de la Hoz Restrepo, Strengert and Krause. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Fernández Villalobos, Nathalie Verónica
Kessel, Barbora
Torres Páez, Johanna Carolina
Strömpl, Julia
Kerrinnes, Tobias
de la Hoz Restrepo, Fernando Pio
Strengert, Monika
Krause, Gérard
Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study
title Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study
title_full Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study
title_fullStr Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study
title_full_unstemmed Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study
title_short Seroprevalence of Hepatitis E virus in children and adolescents living in urban Bogotá: An explorative cross-sectional study
title_sort seroprevalence of hepatitis e virus in children and adolescents living in urban bogotá: an explorative cross-sectional study
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943700/
https://www.ncbi.nlm.nih.gov/pubmed/36844812
http://dx.doi.org/10.3389/fpubh.2023.981172
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