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A next-generation sequencing approach for the detection of mixed species in canned tuna

Tuna cans are relevant seafood products for which mixtures of different tuna species are not allowed according to European regulations. In order to support the prevention of food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b and control region m...

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Autores principales: Klapper, Regina, Velasco, Amaya, Döring, Maik, Schröder, Ute, Sotelo, Carmen G., Brinks, Erik, Muñoz-Colmenero, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943852/
https://www.ncbi.nlm.nih.gov/pubmed/36845509
http://dx.doi.org/10.1016/j.fochx.2023.100560
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author Klapper, Regina
Velasco, Amaya
Döring, Maik
Schröder, Ute
Sotelo, Carmen G.
Brinks, Erik
Muñoz-Colmenero, Marta
author_facet Klapper, Regina
Velasco, Amaya
Döring, Maik
Schröder, Ute
Sotelo, Carmen G.
Brinks, Erik
Muñoz-Colmenero, Marta
author_sort Klapper, Regina
collection PubMed
description Tuna cans are relevant seafood products for which mixtures of different tuna species are not allowed according to European regulations. In order to support the prevention of food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b and control region markers has been tested. Analyses of defined mixtures of DNA, fresh tissue and canned tissue revealed a qualitative and, to some extent, semiquantitative identification of tuna species. While the choice of the bioinformatic pipeline had no influence in the results (p = 0.71), quantitative differences occurred depending on the treatment of the sample, marker, species, and mixture (p < 0.01). The results revealed that matrix-specific calibrators or normalization models should also be used in NGS. The method represents an important step towards a semiquantitative method for routine control of this analytically challenging food matrix. Tests of commercial samples uncovered mixed species in some cans, being not in compliance with EU regulations.
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spelling pubmed-99438522023-02-23 A next-generation sequencing approach for the detection of mixed species in canned tuna Klapper, Regina Velasco, Amaya Döring, Maik Schröder, Ute Sotelo, Carmen G. Brinks, Erik Muñoz-Colmenero, Marta Food Chem X Article(s) from the Special Issue on Food Authentication and Origin by Dr. Yong Fang and Dr. Vural Gökmen Tuna cans are relevant seafood products for which mixtures of different tuna species are not allowed according to European regulations. In order to support the prevention of food fraud and mislabelling, a next-generation sequencing methodology based on mitochondrial cytochrome b and control region markers has been tested. Analyses of defined mixtures of DNA, fresh tissue and canned tissue revealed a qualitative and, to some extent, semiquantitative identification of tuna species. While the choice of the bioinformatic pipeline had no influence in the results (p = 0.71), quantitative differences occurred depending on the treatment of the sample, marker, species, and mixture (p < 0.01). The results revealed that matrix-specific calibrators or normalization models should also be used in NGS. The method represents an important step towards a semiquantitative method for routine control of this analytically challenging food matrix. Tests of commercial samples uncovered mixed species in some cans, being not in compliance with EU regulations. Elsevier 2023-01-05 /pmc/articles/PMC9943852/ /pubmed/36845509 http://dx.doi.org/10.1016/j.fochx.2023.100560 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article(s) from the Special Issue on Food Authentication and Origin by Dr. Yong Fang and Dr. Vural Gökmen
Klapper, Regina
Velasco, Amaya
Döring, Maik
Schröder, Ute
Sotelo, Carmen G.
Brinks, Erik
Muñoz-Colmenero, Marta
A next-generation sequencing approach for the detection of mixed species in canned tuna
title A next-generation sequencing approach for the detection of mixed species in canned tuna
title_full A next-generation sequencing approach for the detection of mixed species in canned tuna
title_fullStr A next-generation sequencing approach for the detection of mixed species in canned tuna
title_full_unstemmed A next-generation sequencing approach for the detection of mixed species in canned tuna
title_short A next-generation sequencing approach for the detection of mixed species in canned tuna
title_sort next-generation sequencing approach for the detection of mixed species in canned tuna
topic Article(s) from the Special Issue on Food Authentication and Origin by Dr. Yong Fang and Dr. Vural Gökmen
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943852/
https://www.ncbi.nlm.nih.gov/pubmed/36845509
http://dx.doi.org/10.1016/j.fochx.2023.100560
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