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One-step CRISPR-Cas9-mediated knockout of native TCRαβ genes in human T cells using RNA electroporation
To avoid mispairing between native and introduced T cell receptors (TCRs) and to prevent graft-versus-host disease in allogeneic T cell therapies, TCRα and TCRβ chains of native TCRs are knocked out via CRISPR-Cas9. We demonstrate the isolation and activation of CD8(+) T cells followed by electropor...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943861/ https://www.ncbi.nlm.nih.gov/pubmed/36853667 http://dx.doi.org/10.1016/j.xpro.2023.102112 |