Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest...

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Detalles Bibliográficos
Autores principales: Zhuo, Meilian, Mao, Jiangjiao, David, Charles J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943862/
https://www.ncbi.nlm.nih.gov/pubmed/36853729
http://dx.doi.org/10.1016/j.xpro.2023.102108
Descripción
Sumario:We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest in the pancreas. The method can also be used to express cDNAs in the pancreas. This method shows great potential to accelerate pancreatic cancer research in autochthonous models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).(1)

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