Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest...

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Detalles Bibliográficos
Autores principales: Zhuo, Meilian, Mao, Jiangjiao, David, Charles J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943862/
https://www.ncbi.nlm.nih.gov/pubmed/36853729
http://dx.doi.org/10.1016/j.xpro.2023.102108
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author Zhuo, Meilian
Mao, Jiangjiao
David, Charles J.
author_facet Zhuo, Meilian
Mao, Jiangjiao
David, Charles J.
author_sort Zhuo, Meilian
collection PubMed
description We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest in the pancreas. The method can also be used to express cDNAs in the pancreas. This method shows great potential to accelerate pancreatic cancer research in autochthonous models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).(1)
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spelling pubmed-99438622023-02-23 Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer Zhuo, Meilian Mao, Jiangjiao David, Charles J. STAR Protoc Protocol We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest in the pancreas. The method can also be used to express cDNAs in the pancreas. This method shows great potential to accelerate pancreatic cancer research in autochthonous models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).(1) Elsevier 2023-02-10 /pmc/articles/PMC9943862/ /pubmed/36853729 http://dx.doi.org/10.1016/j.xpro.2023.102108 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhuo, Meilian
Mao, Jiangjiao
David, Charles J.
Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
title Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
title_full Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
title_fullStr Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
title_full_unstemmed Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
title_short Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
title_sort protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943862/
https://www.ncbi.nlm.nih.gov/pubmed/36853729
http://dx.doi.org/10.1016/j.xpro.2023.102108
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AT davidcharlesj protocoltopackageandconcentrateadenoassociatedvirusserotype8foruseinautochthonousmousemodelsofpancreaticcancer

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