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Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest...

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Detalles Bibliográficos
Autores principales:	Zhuo, Meilian, Mao, Jiangjiao, David, Charles J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9943862/ https://www.ncbi.nlm.nih.gov/pubmed/36853729 http://dx.doi.org/10.1016/j.xpro.2023.102108

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