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Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry
Entirely biological human tissue-engineered blood vessels (TEBV) were previously developed for clinical use. Tissue-engineered models have also proven to be valuable tools in disease modelling. Moreover, there is a need for complex geometry TEBV for study of multifactorial vascular pathologies, such...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944280/ https://www.ncbi.nlm.nih.gov/pubmed/36810756 http://dx.doi.org/10.1038/s41598-023-29825-0 |
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author | Brodeur, Alyssa Winter, Alexandre Roy, Vincent Touzel Deschênes, Lydia Gros-Louis, François Ruel, Jean |
author_facet | Brodeur, Alyssa Winter, Alexandre Roy, Vincent Touzel Deschênes, Lydia Gros-Louis, François Ruel, Jean |
author_sort | Brodeur, Alyssa |
collection | PubMed |
description | Entirely biological human tissue-engineered blood vessels (TEBV) were previously developed for clinical use. Tissue-engineered models have also proven to be valuable tools in disease modelling. Moreover, there is a need for complex geometry TEBV for study of multifactorial vascular pathologies, such as intracranial aneurysms. The main goal of the work reported in this article was to produce an entirely human branched small-caliber TEBV. The use of a novel spherical rotary cell seeding system allows effective and uniform dynamic cell seeding for a viable in vitro tissue-engineered model. In this report, the design and fabrication of an innovative seeding system with random spherical 360° rotation is described. Custom made seeding chambers are placed inside the system and hold Y-shaped polyethylene terephthalate glycol (PETG) scaffolds. The seeding conditions, such as cell concentration, seeding speed and incubation time were optimized via count of cells adhered on the PETG scaffolds. This spheric seeding method was compared to other approaches, such as dynamic and static seeding, and clearly shows uniform cell distribution on PETG scaffolds. With this simple to use spherical system, fully biological branched TEBV constructs were also produced by seeding human fibroblasts directly on custom-made complex geometry PETG mandrels. The production of patient-derived small-caliber TEBVs with complex geometry and optimized cellular distribution all along the vascular reconstructed may be an innovative way to model various vascular diseases such as intracranial aneurysms. |
format | Online Article Text |
id | pubmed-9944280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-99442802023-02-23 Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry Brodeur, Alyssa Winter, Alexandre Roy, Vincent Touzel Deschênes, Lydia Gros-Louis, François Ruel, Jean Sci Rep Article Entirely biological human tissue-engineered blood vessels (TEBV) were previously developed for clinical use. Tissue-engineered models have also proven to be valuable tools in disease modelling. Moreover, there is a need for complex geometry TEBV for study of multifactorial vascular pathologies, such as intracranial aneurysms. The main goal of the work reported in this article was to produce an entirely human branched small-caliber TEBV. The use of a novel spherical rotary cell seeding system allows effective and uniform dynamic cell seeding for a viable in vitro tissue-engineered model. In this report, the design and fabrication of an innovative seeding system with random spherical 360° rotation is described. Custom made seeding chambers are placed inside the system and hold Y-shaped polyethylene terephthalate glycol (PETG) scaffolds. The seeding conditions, such as cell concentration, seeding speed and incubation time were optimized via count of cells adhered on the PETG scaffolds. This spheric seeding method was compared to other approaches, such as dynamic and static seeding, and clearly shows uniform cell distribution on PETG scaffolds. With this simple to use spherical system, fully biological branched TEBV constructs were also produced by seeding human fibroblasts directly on custom-made complex geometry PETG mandrels. The production of patient-derived small-caliber TEBVs with complex geometry and optimized cellular distribution all along the vascular reconstructed may be an innovative way to model various vascular diseases such as intracranial aneurysms. Nature Publishing Group UK 2023-02-21 /pmc/articles/PMC9944280/ /pubmed/36810756 http://dx.doi.org/10.1038/s41598-023-29825-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Brodeur, Alyssa Winter, Alexandre Roy, Vincent Touzel Deschênes, Lydia Gros-Louis, François Ruel, Jean Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
title | Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
title_full | Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
title_fullStr | Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
title_full_unstemmed | Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
title_short | Spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
title_sort | spherical rotary cell seeding system for production of small-caliber tissue-engineered blood vessels with complex geometry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944280/ https://www.ncbi.nlm.nih.gov/pubmed/36810756 http://dx.doi.org/10.1038/s41598-023-29825-0 |
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