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The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury

Introduction: Acute lung injury (ALI) initiates an inflammatory cascade that impairs gas exchange, induces hypoxemia, and causes an increase in respiratory rate (f(R)). This stimulates the carotid body (CB) chemoreflex, a fundamental protective reflex that maintains oxygen homeostasis. Our previous...

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Autores principales: Kamra, Kajal, Karpuk, Nikolay, Zucker, Irving H., Schultz, Harold D., Wang, Han-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944401/
https://www.ncbi.nlm.nih.gov/pubmed/36846321
http://dx.doi.org/10.3389/fphys.2023.1101408
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author Kamra, Kajal
Karpuk, Nikolay
Zucker, Irving H.
Schultz, Harold D.
Wang, Han-Jun
author_facet Kamra, Kajal
Karpuk, Nikolay
Zucker, Irving H.
Schultz, Harold D.
Wang, Han-Jun
author_sort Kamra, Kajal
collection PubMed
description Introduction: Acute lung injury (ALI) initiates an inflammatory cascade that impairs gas exchange, induces hypoxemia, and causes an increase in respiratory rate (f(R)). This stimulates the carotid body (CB) chemoreflex, a fundamental protective reflex that maintains oxygen homeostasis. Our previous study indicated that the chemoreflex is sensitized during the recovery from ALI. The superior cervical ganglion (SCG) is known to innervate the CB, and its electrical stimulation has been shown to significantly sensitize the chemoreflex in hypertensive and normotensive rats. We hypothesized that the SCG is involved in the chemoreflex sensitization post-ALI. Methods: We performed a bilateral SCG ganglionectomy (SCGx) or sham-SCGx (Sx) in male Sprague Dawley rats 2 weeks before inducing ALI (Week −2 i.e., W-2). ALI was induced using a single intra-tracheal instillation of bleomycin (bleo) (day 1). Resting-f(R), V(t) (Tidal Volume), and V̇ (E) (Minute Ventilation) were measured. The chemoreflex response to hypoxia (10% O(2), 0% CO(2)) and normoxic-hypercapnia (21% O(2), 5% CO(2)) were measured before surgery on W (−3), before bleo administration on W0 and on W4 post-bleo using whole-body plethysmography (WBP). Results: SCGx did not affect resting f(R), V(t) and V̇(E) as well as the chemoreflex responses to hypoxia and normoxic hypercapnia in either group prior to bleo. There was no significant difference in ALI-induced increase in resting f(R) between Sx and SCGx rats at W1 post-bleo. At W4 post-bleo, there were no significant differences in resting f(R), V(t), and V̇(E) between Sx and SCGx rats. Consistent with our previous study, we observed a sensitized chemoreflex (delta f(R)) in response to hypoxia and normoxic hypercapnia in Sx rats at W4 post-bleo. However, at the same time, compared to Sx rats, the chemoreflex sensitivity was significantly less in SCGx rats in response to either hypoxia or normoxic hypercapnia. Discussion: These data suggest that SCG is involved in the chemoreflex sensitization during ALI recovery. Further understanding of the underlying mechanism will provide important information for the long-term goal of developing novel targeted therapeutic approaches to pulmonary diseases to improve clinical outcomes.
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spelling pubmed-99444012023-02-23 The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury Kamra, Kajal Karpuk, Nikolay Zucker, Irving H. Schultz, Harold D. Wang, Han-Jun Front Physiol Physiology Introduction: Acute lung injury (ALI) initiates an inflammatory cascade that impairs gas exchange, induces hypoxemia, and causes an increase in respiratory rate (f(R)). This stimulates the carotid body (CB) chemoreflex, a fundamental protective reflex that maintains oxygen homeostasis. Our previous study indicated that the chemoreflex is sensitized during the recovery from ALI. The superior cervical ganglion (SCG) is known to innervate the CB, and its electrical stimulation has been shown to significantly sensitize the chemoreflex in hypertensive and normotensive rats. We hypothesized that the SCG is involved in the chemoreflex sensitization post-ALI. Methods: We performed a bilateral SCG ganglionectomy (SCGx) or sham-SCGx (Sx) in male Sprague Dawley rats 2 weeks before inducing ALI (Week −2 i.e., W-2). ALI was induced using a single intra-tracheal instillation of bleomycin (bleo) (day 1). Resting-f(R), V(t) (Tidal Volume), and V̇ (E) (Minute Ventilation) were measured. The chemoreflex response to hypoxia (10% O(2), 0% CO(2)) and normoxic-hypercapnia (21% O(2), 5% CO(2)) were measured before surgery on W (−3), before bleo administration on W0 and on W4 post-bleo using whole-body plethysmography (WBP). Results: SCGx did not affect resting f(R), V(t) and V̇(E) as well as the chemoreflex responses to hypoxia and normoxic hypercapnia in either group prior to bleo. There was no significant difference in ALI-induced increase in resting f(R) between Sx and SCGx rats at W1 post-bleo. At W4 post-bleo, there were no significant differences in resting f(R), V(t), and V̇(E) between Sx and SCGx rats. Consistent with our previous study, we observed a sensitized chemoreflex (delta f(R)) in response to hypoxia and normoxic hypercapnia in Sx rats at W4 post-bleo. However, at the same time, compared to Sx rats, the chemoreflex sensitivity was significantly less in SCGx rats in response to either hypoxia or normoxic hypercapnia. Discussion: These data suggest that SCG is involved in the chemoreflex sensitization during ALI recovery. Further understanding of the underlying mechanism will provide important information for the long-term goal of developing novel targeted therapeutic approaches to pulmonary diseases to improve clinical outcomes. Frontiers Media S.A. 2023-02-08 /pmc/articles/PMC9944401/ /pubmed/36846321 http://dx.doi.org/10.3389/fphys.2023.1101408 Text en Copyright © 2023 Kamra, Karpuk, Zucker, Schultz and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Kamra, Kajal
Karpuk, Nikolay
Zucker, Irving H.
Schultz, Harold D.
Wang, Han-Jun
The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
title The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
title_full The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
title_fullStr The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
title_full_unstemmed The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
title_short The superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
title_sort superior cervical ganglion is involved in chronic chemoreflex sensitization during recovery from acute lung injury
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944401/
https://www.ncbi.nlm.nih.gov/pubmed/36846321
http://dx.doi.org/10.3389/fphys.2023.1101408
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