Removal of substrate inhibition of Acinetobacter baumannii xanthine oxidase by point mutation at Gln-201 enables efficient reduction of purine content in fish sauce

Xanthine oxidase is an oxidase that has a molybdopterin structure with substrate inhibition. Here, we show that a single point mutation (Q201) in the Acinetobacter baumannii xanthine oxidase (AbXOD) obtained mutant Q201E (k(cat) =799.44 s(−1), no inhibition) with high enzyme activity and decrease of...

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Detalles Bibliográficos
Autores principales: Wen, You, Xu, Jiahui, Pan, Donglei, Wang, Chenghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944496/
https://www.ncbi.nlm.nih.gov/pubmed/36845495
http://dx.doi.org/10.1016/j.fochx.2023.100593

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944496/
https://www.ncbi.nlm.nih.gov/pubmed/36845495
http://dx.doi.org/10.1016/j.fochx.2023.100593

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