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Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions

In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic f...

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Autores principales: Pérez-Piñeiro, Javier, Sánchez-Cea, Fernando, Arce, Mariana P., Lado-Touriño, Isabel, Rojas-Cervantes, María Luisa, Gilsanz, María Fuencisla, Gallach-Pérez, Darío, Blasco, Rodrigo, Barrios-Bermúdez, Niurka, Cerpa-Naranjo, Arisbel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944857/
https://www.ncbi.nlm.nih.gov/pubmed/36810433
http://dx.doi.org/10.3390/jox13010008
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author Pérez-Piñeiro, Javier
Sánchez-Cea, Fernando
Arce, Mariana P.
Lado-Touriño, Isabel
Rojas-Cervantes, María Luisa
Gilsanz, María Fuencisla
Gallach-Pérez, Darío
Blasco, Rodrigo
Barrios-Bermúdez, Niurka
Cerpa-Naranjo, Arisbel
author_facet Pérez-Piñeiro, Javier
Sánchez-Cea, Fernando
Arce, Mariana P.
Lado-Touriño, Isabel
Rojas-Cervantes, María Luisa
Gilsanz, María Fuencisla
Gallach-Pérez, Darío
Blasco, Rodrigo
Barrios-Bermúdez, Niurka
Cerpa-Naranjo, Arisbel
author_sort Pérez-Piñeiro, Javier
collection PubMed
description In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic force microscopy, and ultraviolet visible spectroscopy, comparing the starting nanomaterials with the nanomaterials in contact with the biological material, i.e., bovine fetal serum. The different experiments were performed at different concentrations of nanomaterial (0.125–0.5 mg/mL) and BSA (0.01–0.04 mg/mL), at different incubation times (5–360 min), with and without PEG, and at different temperatures (25–40 °C). The SEM results show that BSA is adsorbed on the surface of the graphene oxide nanomaterial. Using UV-Vis spectrophotometry, the characteristic absorption peaks of BSA are observed at 210 and 280 nm, corroborating that the protein has been adsorbed. When the time increases, the BSA protein can be detached from the nanomaterial due to a desorption process. The stability of the dispersions is reached at a pH between 7 and 9. The dispersions behave like a Newtonian fluid with viscosity values between 1.1 and 1.5 mPa·s at a temperature range of 25 to 40 °C. The viscosity values decrease as the temperature increases.
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spelling pubmed-99448572023-02-23 Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions Pérez-Piñeiro, Javier Sánchez-Cea, Fernando Arce, Mariana P. Lado-Touriño, Isabel Rojas-Cervantes, María Luisa Gilsanz, María Fuencisla Gallach-Pérez, Darío Blasco, Rodrigo Barrios-Bermúdez, Niurka Cerpa-Naranjo, Arisbel J Xenobiot Article In this work, a stability study of dispersions of graphene oxide and graphene oxide functionalized with polyethylene glycol (PEG) in the presence of bovine serum albumin is carried out. First, a structural characterization of these nanomaterials is performed by scanning electron microscopy, atomic force microscopy, and ultraviolet visible spectroscopy, comparing the starting nanomaterials with the nanomaterials in contact with the biological material, i.e., bovine fetal serum. The different experiments were performed at different concentrations of nanomaterial (0.125–0.5 mg/mL) and BSA (0.01–0.04 mg/mL), at different incubation times (5–360 min), with and without PEG, and at different temperatures (25–40 °C). The SEM results show that BSA is adsorbed on the surface of the graphene oxide nanomaterial. Using UV-Vis spectrophotometry, the characteristic absorption peaks of BSA are observed at 210 and 280 nm, corroborating that the protein has been adsorbed. When the time increases, the BSA protein can be detached from the nanomaterial due to a desorption process. The stability of the dispersions is reached at a pH between 7 and 9. The dispersions behave like a Newtonian fluid with viscosity values between 1.1 and 1.5 mPa·s at a temperature range of 25 to 40 °C. The viscosity values decrease as the temperature increases. MDPI 2023-02-16 /pmc/articles/PMC9944857/ /pubmed/36810433 http://dx.doi.org/10.3390/jox13010008 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pérez-Piñeiro, Javier
Sánchez-Cea, Fernando
Arce, Mariana P.
Lado-Touriño, Isabel
Rojas-Cervantes, María Luisa
Gilsanz, María Fuencisla
Gallach-Pérez, Darío
Blasco, Rodrigo
Barrios-Bermúdez, Niurka
Cerpa-Naranjo, Arisbel
Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
title Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
title_full Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
title_fullStr Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
title_full_unstemmed Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
title_short Stability Study of Graphene Oxide-Bovine Serum Albumin Dispersions
title_sort stability study of graphene oxide-bovine serum albumin dispersions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944857/
https://www.ncbi.nlm.nih.gov/pubmed/36810433
http://dx.doi.org/10.3390/jox13010008
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