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Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy
INTRODUCTION: Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers. MATERIAL AND METHODS: Tear films were collected on Schirmer strips f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Sciendo
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944995/ https://www.ncbi.nlm.nih.gov/pubmed/36846040 http://dx.doi.org/10.2478/jvetres-2022-0053 |
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author | Winiarczyk, Dagmara Winiarczyk, Mateusz Balicki, Ireneusz Szadkowski, Mateusz Michalak, Katarzyna Winiarczyk, Stanisław Adaszek, Łukasz |
author_facet | Winiarczyk, Dagmara Winiarczyk, Mateusz Balicki, Ireneusz Szadkowski, Mateusz Michalak, Katarzyna Winiarczyk, Stanisław Adaszek, Łukasz |
author_sort | Winiarczyk, Dagmara |
collection | PubMed |
description | INTRODUCTION: Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers. MATERIAL AND METHODS: Tear films were collected on Schirmer strips from 32 canine patients (12 diabetic dogs without changes in the retina, 8 diabetic dogs with signs of DR, and 12 control dogs). Two-dimensional electrophoresis was used to separate tear film proteins prior to their identification with matrix-assisted laser desorption/ionisation–tandem time-of-flight mass spectrometry and interrogation of protein function databases to find matches. RESULTS: Five significantly differentially expressed proteins were identified; of those, one was downregulated (2ʹ-5ʹ-oligoadenylate synthase 3) and four were upregulated in the tear film of two diabetic groups (Ras-related protein RAB-13; aldo-keto-reductase family 1 member C3; 28S ribosomal protein S31, mitochondrial; and 60S ribosomal protein L5). The differentially expressed proteins identified in the tear film were involved in signalling pathways associated with impaired protein clearance, persistent inflammation and oxidative stress. CONCLUSION: The results of our study confirm that the pathological process in the retina in the course of diabetes mellitus causes changes in the tear film proteome. |
format | Online Article Text |
id | pubmed-9944995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-99449952023-02-23 Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy Winiarczyk, Dagmara Winiarczyk, Mateusz Balicki, Ireneusz Szadkowski, Mateusz Michalak, Katarzyna Winiarczyk, Stanisław Adaszek, Łukasz J Vet Res Research Articles INTRODUCTION: Diabetic retinopathy (DR) is the leading cause of blindness in human and animal patients. Early detection and treatment of the disease are important and can be facilitated by proteomic approaches providing biomarkers. MATERIAL AND METHODS: Tear films were collected on Schirmer strips from 32 canine patients (12 diabetic dogs without changes in the retina, 8 diabetic dogs with signs of DR, and 12 control dogs). Two-dimensional electrophoresis was used to separate tear film proteins prior to their identification with matrix-assisted laser desorption/ionisation–tandem time-of-flight mass spectrometry and interrogation of protein function databases to find matches. RESULTS: Five significantly differentially expressed proteins were identified; of those, one was downregulated (2ʹ-5ʹ-oligoadenylate synthase 3) and four were upregulated in the tear film of two diabetic groups (Ras-related protein RAB-13; aldo-keto-reductase family 1 member C3; 28S ribosomal protein S31, mitochondrial; and 60S ribosomal protein L5). The differentially expressed proteins identified in the tear film were involved in signalling pathways associated with impaired protein clearance, persistent inflammation and oxidative stress. CONCLUSION: The results of our study confirm that the pathological process in the retina in the course of diabetes mellitus causes changes in the tear film proteome. Sciendo 2022-11-07 /pmc/articles/PMC9944995/ /pubmed/36846040 http://dx.doi.org/10.2478/jvetres-2022-0053 Text en © 2022 D. Winiarczyk et al. published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Articles Winiarczyk, Dagmara Winiarczyk, Mateusz Balicki, Ireneusz Szadkowski, Mateusz Michalak, Katarzyna Winiarczyk, Stanisław Adaszek, Łukasz Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy |
title | Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy |
title_full | Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy |
title_fullStr | Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy |
title_full_unstemmed | Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy |
title_short | Proteomic Analysis of Tear Film in Canine Diabetic Patients with And Without Retinopathy |
title_sort | proteomic analysis of tear film in canine diabetic patients with and without retinopathy |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9944995/ https://www.ncbi.nlm.nih.gov/pubmed/36846040 http://dx.doi.org/10.2478/jvetres-2022-0053 |
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