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Grouper cGAS is a negative regulator of STING-mediated interferon response
Cyclic GMP-AMP synthase (cGAS) is one of the classical pattern recognition receptors that recognizes mainly intracytoplasmic DNA. cGAS induces type I IFN responses to the cGAS-STING signaling pathway. To investigate the roles of cGAS-STING signaling pathway in grouper, a cGAS homolog (named EccGAS)...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945316/ https://www.ncbi.nlm.nih.gov/pubmed/36845102 http://dx.doi.org/10.3389/fimmu.2023.1092824 |
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author | Zhang, Luhao Zhang, Xin Liao, Jiaming Xu, Linting Kang, Shaozhu Chen, Hong Sun, Mengshi Wu, Siting Xu, Zhuqing Wei, Shina Qin, Qiwei Wei, Jingguang |
author_facet | Zhang, Luhao Zhang, Xin Liao, Jiaming Xu, Linting Kang, Shaozhu Chen, Hong Sun, Mengshi Wu, Siting Xu, Zhuqing Wei, Shina Qin, Qiwei Wei, Jingguang |
author_sort | Zhang, Luhao |
collection | PubMed |
description | Cyclic GMP-AMP synthase (cGAS) is one of the classical pattern recognition receptors that recognizes mainly intracytoplasmic DNA. cGAS induces type I IFN responses to the cGAS-STING signaling pathway. To investigate the roles of cGAS-STING signaling pathway in grouper, a cGAS homolog (named EccGAS) was cloned and identified from orange-spotted grouper (Epinephelus coioides). The open reading frame (ORF) of EccGAS is 1695 bp, encodes 575 amino acids, and contains a Mab-21 typical structural domain. EccGAS is homologous to Sebastes umbrosus and humans at 71.8% and 41.49%, respectively. EccGAS mRNA is abundant in the blood, skin, and gills. It is uniformly distributed in the cytoplasm and colocalized in the endoplasmic reticulum and mitochondria. Silencing of EccGAS inhibited the replication of Singapore grouper iridovirus (SGIV) in grouper spleen (GS) cells and enhanced the expression of interferon-related factors. Furthermore, EccGAS inhibited EcSTING-mediated interferon response and interacted with EcSTING, EcTAK1, EcTBK1, and EcIRF3. These results suggest that EccGAS may be a negative regulator of the cGAS-STING signaling pathway of fish. |
format | Online Article Text |
id | pubmed-9945316 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99453162023-02-23 Grouper cGAS is a negative regulator of STING-mediated interferon response Zhang, Luhao Zhang, Xin Liao, Jiaming Xu, Linting Kang, Shaozhu Chen, Hong Sun, Mengshi Wu, Siting Xu, Zhuqing Wei, Shina Qin, Qiwei Wei, Jingguang Front Immunol Immunology Cyclic GMP-AMP synthase (cGAS) is one of the classical pattern recognition receptors that recognizes mainly intracytoplasmic DNA. cGAS induces type I IFN responses to the cGAS-STING signaling pathway. To investigate the roles of cGAS-STING signaling pathway in grouper, a cGAS homolog (named EccGAS) was cloned and identified from orange-spotted grouper (Epinephelus coioides). The open reading frame (ORF) of EccGAS is 1695 bp, encodes 575 amino acids, and contains a Mab-21 typical structural domain. EccGAS is homologous to Sebastes umbrosus and humans at 71.8% and 41.49%, respectively. EccGAS mRNA is abundant in the blood, skin, and gills. It is uniformly distributed in the cytoplasm and colocalized in the endoplasmic reticulum and mitochondria. Silencing of EccGAS inhibited the replication of Singapore grouper iridovirus (SGIV) in grouper spleen (GS) cells and enhanced the expression of interferon-related factors. Furthermore, EccGAS inhibited EcSTING-mediated interferon response and interacted with EcSTING, EcTAK1, EcTBK1, and EcIRF3. These results suggest that EccGAS may be a negative regulator of the cGAS-STING signaling pathway of fish. Frontiers Media S.A. 2023-02-07 /pmc/articles/PMC9945316/ /pubmed/36845102 http://dx.doi.org/10.3389/fimmu.2023.1092824 Text en Copyright © 2023 Zhang, Zhang, Liao, Xu, Kang, Chen, Sun, Wu, Xu, Wei, Qin and Wei https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Zhang, Luhao Zhang, Xin Liao, Jiaming Xu, Linting Kang, Shaozhu Chen, Hong Sun, Mengshi Wu, Siting Xu, Zhuqing Wei, Shina Qin, Qiwei Wei, Jingguang Grouper cGAS is a negative regulator of STING-mediated interferon response |
title | Grouper cGAS is a negative regulator of STING-mediated interferon response |
title_full | Grouper cGAS is a negative regulator of STING-mediated interferon response |
title_fullStr | Grouper cGAS is a negative regulator of STING-mediated interferon response |
title_full_unstemmed | Grouper cGAS is a negative regulator of STING-mediated interferon response |
title_short | Grouper cGAS is a negative regulator of STING-mediated interferon response |
title_sort | grouper cgas is a negative regulator of sting-mediated interferon response |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945316/ https://www.ncbi.nlm.nih.gov/pubmed/36845102 http://dx.doi.org/10.3389/fimmu.2023.1092824 |
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