Cargando…

From prediction to function: Current practices and challenges towards the functional characterization of type III effectors

The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell’s immunity and to establish a niche. Here we look at the differ...

Descripción completa

Detalles Bibliográficos
Autores principales: De Ryck, Joren, Van Damme, Petra, Goormachtig, Sofie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945535/
https://www.ncbi.nlm.nih.gov/pubmed/36846751
http://dx.doi.org/10.3389/fmicb.2023.1113442
_version_ 1784892160051511296
author De Ryck, Joren
Van Damme, Petra
Goormachtig, Sofie
author_facet De Ryck, Joren
Van Damme, Petra
Goormachtig, Sofie
author_sort De Ryck, Joren
collection PubMed
description The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell’s immunity and to establish a niche. Here we look at the different approaches that are used to functionally characterize a T3E. Such approaches include host localization studies, virulence screenings, biochemical activity assays, and large-scale omics, such as transcriptomics, interactomics, and metabolomics, among others. By means of the phytopathogenic Ralstonia solanacearum species complex (RSSC) as a case study, the current advances of these methods will be explored, alongside the progress made in understanding effector biology. Data obtained by such complementary methods provide crucial information to comprehend the entire function of the effectome and will eventually lead to a better understanding of the phytopathogen, opening opportunities to tackle it.
format Online
Article
Text
id pubmed-9945535
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99455352023-02-23 From prediction to function: Current practices and challenges towards the functional characterization of type III effectors De Ryck, Joren Van Damme, Petra Goormachtig, Sofie Front Microbiol Microbiology The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell’s immunity and to establish a niche. Here we look at the different approaches that are used to functionally characterize a T3E. Such approaches include host localization studies, virulence screenings, biochemical activity assays, and large-scale omics, such as transcriptomics, interactomics, and metabolomics, among others. By means of the phytopathogenic Ralstonia solanacearum species complex (RSSC) as a case study, the current advances of these methods will be explored, alongside the progress made in understanding effector biology. Data obtained by such complementary methods provide crucial information to comprehend the entire function of the effectome and will eventually lead to a better understanding of the phytopathogen, opening opportunities to tackle it. Frontiers Media S.A. 2023-02-08 /pmc/articles/PMC9945535/ /pubmed/36846751 http://dx.doi.org/10.3389/fmicb.2023.1113442 Text en Copyright © 2023 De Ryck, Van Damme and Goormachtig. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
De Ryck, Joren
Van Damme, Petra
Goormachtig, Sofie
From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
title From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
title_full From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
title_fullStr From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
title_full_unstemmed From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
title_short From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
title_sort from prediction to function: current practices and challenges towards the functional characterization of type iii effectors
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945535/
https://www.ncbi.nlm.nih.gov/pubmed/36846751
http://dx.doi.org/10.3389/fmicb.2023.1113442
work_keys_str_mv AT deryckjoren frompredictiontofunctioncurrentpracticesandchallengestowardsthefunctionalcharacterizationoftypeiiieffectors
AT vandammepetra frompredictiontofunctioncurrentpracticesandchallengestowardsthefunctionalcharacterizationoftypeiiieffectors
AT goormachtigsofie frompredictiontofunctioncurrentpracticesandchallengestowardsthefunctionalcharacterizationoftypeiiieffectors