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From prediction to function: Current practices and challenges towards the functional characterization of type III effectors
The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell’s immunity and to establish a niche. Here we look at the differ...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945535/ https://www.ncbi.nlm.nih.gov/pubmed/36846751 http://dx.doi.org/10.3389/fmicb.2023.1113442 |
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author | De Ryck, Joren Van Damme, Petra Goormachtig, Sofie |
author_facet | De Ryck, Joren Van Damme, Petra Goormachtig, Sofie |
author_sort | De Ryck, Joren |
collection | PubMed |
description | The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell’s immunity and to establish a niche. Here we look at the different approaches that are used to functionally characterize a T3E. Such approaches include host localization studies, virulence screenings, biochemical activity assays, and large-scale omics, such as transcriptomics, interactomics, and metabolomics, among others. By means of the phytopathogenic Ralstonia solanacearum species complex (RSSC) as a case study, the current advances of these methods will be explored, alongside the progress made in understanding effector biology. Data obtained by such complementary methods provide crucial information to comprehend the entire function of the effectome and will eventually lead to a better understanding of the phytopathogen, opening opportunities to tackle it. |
format | Online Article Text |
id | pubmed-9945535 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99455352023-02-23 From prediction to function: Current practices and challenges towards the functional characterization of type III effectors De Ryck, Joren Van Damme, Petra Goormachtig, Sofie Front Microbiol Microbiology The type III secretion system (T3SS) is a well-studied pathogenicity determinant of many bacteria through which effectors (T3Es) are translocated into the host cell, where they exercise a wide range of functions to deceive the host cell’s immunity and to establish a niche. Here we look at the different approaches that are used to functionally characterize a T3E. Such approaches include host localization studies, virulence screenings, biochemical activity assays, and large-scale omics, such as transcriptomics, interactomics, and metabolomics, among others. By means of the phytopathogenic Ralstonia solanacearum species complex (RSSC) as a case study, the current advances of these methods will be explored, alongside the progress made in understanding effector biology. Data obtained by such complementary methods provide crucial information to comprehend the entire function of the effectome and will eventually lead to a better understanding of the phytopathogen, opening opportunities to tackle it. Frontiers Media S.A. 2023-02-08 /pmc/articles/PMC9945535/ /pubmed/36846751 http://dx.doi.org/10.3389/fmicb.2023.1113442 Text en Copyright © 2023 De Ryck, Van Damme and Goormachtig. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology De Ryck, Joren Van Damme, Petra Goormachtig, Sofie From prediction to function: Current practices and challenges towards the functional characterization of type III effectors |
title | From prediction to function: Current practices and challenges towards the functional characterization of type III effectors |
title_full | From prediction to function: Current practices and challenges towards the functional characterization of type III effectors |
title_fullStr | From prediction to function: Current practices and challenges towards the functional characterization of type III effectors |
title_full_unstemmed | From prediction to function: Current practices and challenges towards the functional characterization of type III effectors |
title_short | From prediction to function: Current practices and challenges towards the functional characterization of type III effectors |
title_sort | from prediction to function: current practices and challenges towards the functional characterization of type iii effectors |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945535/ https://www.ncbi.nlm.nih.gov/pubmed/36846751 http://dx.doi.org/10.3389/fmicb.2023.1113442 |
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