Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020

Aspergillus fumigatus, an environmental mold, causes life-threatening infections. Studies on the phylogenetic structure of human clinical A. fumigatus isolates are limited. Here, we performed whole genome sequencing of 24 A. fumigatus isolates collected from 18 patients in U.S. healthcare facilities...

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Autores principales: Misas, Elizabeth, Deng, John Z, Gold, Jeremy A W, Gade, Lalitha, Nunnally, Natalie S, Georgacopoulos, Ourania, Bentz, Meghan, Berkow, Elizabeth L, Litvintseva, Anastasia P, Chiller, Tom M, Klausner, Jeffrey D, Chow, Nancy A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945844/
https://www.ncbi.nlm.nih.gov/pubmed/36715156
http://dx.doi.org/10.1093/mmy/myad012
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author Misas, Elizabeth
Deng, John Z
Gold, Jeremy A W
Gade, Lalitha
Nunnally, Natalie S
Georgacopoulos, Ourania
Bentz, Meghan
Berkow, Elizabeth L
Litvintseva, Anastasia P
Chiller, Tom M
Klausner, Jeffrey D
Chow, Nancy A
author_facet Misas, Elizabeth
Deng, John Z
Gold, Jeremy A W
Gade, Lalitha
Nunnally, Natalie S
Georgacopoulos, Ourania
Bentz, Meghan
Berkow, Elizabeth L
Litvintseva, Anastasia P
Chiller, Tom M
Klausner, Jeffrey D
Chow, Nancy A
author_sort Misas, Elizabeth
collection PubMed
description Aspergillus fumigatus, an environmental mold, causes life-threatening infections. Studies on the phylogenetic structure of human clinical A. fumigatus isolates are limited. Here, we performed whole genome sequencing of 24 A. fumigatus isolates collected from 18 patients in U.S. healthcare facilities in California. Single-nucleotide polymorphism (SNP) differences between patient isolates ranged from 187 to 70 829 SNPs. For five patients with multiple isolates, we calculated the within-host diversities. Three patients had a within-host diversity that ranged from 4 to 10 SNPs and two patients ranged from 2 to 16 977 SNPs. Findings revealed highly diverse A. fumigatus strains among patients and two patterns of diversity for isolates that come from the same patient, low and extremely high diversity.
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spelling pubmed-99458442023-02-23 Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020 Misas, Elizabeth Deng, John Z Gold, Jeremy A W Gade, Lalitha Nunnally, Natalie S Georgacopoulos, Ourania Bentz, Meghan Berkow, Elizabeth L Litvintseva, Anastasia P Chiller, Tom M Klausner, Jeffrey D Chow, Nancy A Med Mycol Brief Report Aspergillus fumigatus, an environmental mold, causes life-threatening infections. Studies on the phylogenetic structure of human clinical A. fumigatus isolates are limited. Here, we performed whole genome sequencing of 24 A. fumigatus isolates collected from 18 patients in U.S. healthcare facilities in California. Single-nucleotide polymorphism (SNP) differences between patient isolates ranged from 187 to 70 829 SNPs. For five patients with multiple isolates, we calculated the within-host diversities. Three patients had a within-host diversity that ranged from 4 to 10 SNPs and two patients ranged from 2 to 16 977 SNPs. Findings revealed highly diverse A. fumigatus strains among patients and two patterns of diversity for isolates that come from the same patient, low and extremely high diversity. Oxford University Press 2023-01-28 /pmc/articles/PMC9945844/ /pubmed/36715156 http://dx.doi.org/10.1093/mmy/myad012 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Misas, Elizabeth
Deng, John Z
Gold, Jeremy A W
Gade, Lalitha
Nunnally, Natalie S
Georgacopoulos, Ourania
Bentz, Meghan
Berkow, Elizabeth L
Litvintseva, Anastasia P
Chiller, Tom M
Klausner, Jeffrey D
Chow, Nancy A
Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020
title Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020
title_full Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020
title_fullStr Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020
title_full_unstemmed Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020
title_short Genomic description of human clinical Aspergillus fumigatus isolates, California, 2020
title_sort genomic description of human clinical aspergillus fumigatus isolates, california, 2020
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945844/
https://www.ncbi.nlm.nih.gov/pubmed/36715156
http://dx.doi.org/10.1093/mmy/myad012
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