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Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles

The phase transition from solitary to gregarious locusts is crucial in outbreaks of locust plague, which threaten agricultural yield and food security. Research on the regulatory mechanisms of phase transition in locusts has focused primarily on the transcriptional or posttranslational level. Howeve...

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Autores principales: Li, Jing, Wei, Liya, Wang, Yongsheng, Zhang, Haikang, Yang, Pengcheng, Zhao, Zhangwu, Kang, Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945961/
https://www.ncbi.nlm.nih.gov/pubmed/36701367
http://dx.doi.org/10.1073/pnas.2216851120
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author Li, Jing
Wei, Liya
Wang, Yongsheng
Zhang, Haikang
Yang, Pengcheng
Zhao, Zhangwu
Kang, Le
author_facet Li, Jing
Wei, Liya
Wang, Yongsheng
Zhang, Haikang
Yang, Pengcheng
Zhao, Zhangwu
Kang, Le
author_sort Li, Jing
collection PubMed
description The phase transition from solitary to gregarious locusts is crucial in outbreaks of locust plague, which threaten agricultural yield and food security. Research on the regulatory mechanisms of phase transition in locusts has focused primarily on the transcriptional or posttranslational level. However, the translational regulation of phase transition is unexplored. Here, we show a phase-dependent pattern at the translation level, which exhibits different polysome profiles between gregarious and solitary locusts. The gregarious locusts exhibit significant increases in 60S and polyribosomes, while solitary locusts possess higher peaks of the monoribosome and a specific “halfmer.” The polysome profiles, a molecular phenotype, respond to changes in population density. In gregarious locusts, ten genes involved in the cytosolic ribosome pathway exhibited increased translational efficiency (TE). In solitary locusts, five genes from the mitochondrial ribosome pathway displayed increased TE. The high expression of large ribosomal protein 7 at the translational level promotes accumulation of the free 60S ribosomal subunit in gregarious locusts, while solitary locusts employ mitochondrial small ribosomal protein 18c to induce the assembly of mitochondrial ribosomes, causing divergence of the translational profiles and behavioral transition. This study reveals the translational regulatory mechanism of locust phase transition, in which the locusts employ divergent ribosome pathways to cope with changes in population density.
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spelling pubmed-99459612023-02-23 Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles Li, Jing Wei, Liya Wang, Yongsheng Zhang, Haikang Yang, Pengcheng Zhao, Zhangwu Kang, Le Proc Natl Acad Sci U S A Biological Sciences The phase transition from solitary to gregarious locusts is crucial in outbreaks of locust plague, which threaten agricultural yield and food security. Research on the regulatory mechanisms of phase transition in locusts has focused primarily on the transcriptional or posttranslational level. However, the translational regulation of phase transition is unexplored. Here, we show a phase-dependent pattern at the translation level, which exhibits different polysome profiles between gregarious and solitary locusts. The gregarious locusts exhibit significant increases in 60S and polyribosomes, while solitary locusts possess higher peaks of the monoribosome and a specific “halfmer.” The polysome profiles, a molecular phenotype, respond to changes in population density. In gregarious locusts, ten genes involved in the cytosolic ribosome pathway exhibited increased translational efficiency (TE). In solitary locusts, five genes from the mitochondrial ribosome pathway displayed increased TE. The high expression of large ribosomal protein 7 at the translational level promotes accumulation of the free 60S ribosomal subunit in gregarious locusts, while solitary locusts employ mitochondrial small ribosomal protein 18c to induce the assembly of mitochondrial ribosomes, causing divergence of the translational profiles and behavioral transition. This study reveals the translational regulatory mechanism of locust phase transition, in which the locusts employ divergent ribosome pathways to cope with changes in population density. National Academy of Sciences 2023-01-26 2023-01-31 /pmc/articles/PMC9945961/ /pubmed/36701367 http://dx.doi.org/10.1073/pnas.2216851120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
Li, Jing
Wei, Liya
Wang, Yongsheng
Zhang, Haikang
Yang, Pengcheng
Zhao, Zhangwu
Kang, Le
Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
title Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
title_full Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
title_fullStr Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
title_full_unstemmed Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
title_short Cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
title_sort cytosolic and mitochondrial ribosomal proteins mediate the locust phase transition via divergence of translational profiles
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945961/
https://www.ncbi.nlm.nih.gov/pubmed/36701367
http://dx.doi.org/10.1073/pnas.2216851120
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