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Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum

Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic...

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Autores principales: Xie, Hao, Lyratzakis, Alexandros, Khera, Radhika, Koutantou, Myrto, Welsch, Sonja, Michel, Hartmut, Tsiotis, Georgios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945994/
https://www.ncbi.nlm.nih.gov/pubmed/36693097
http://dx.doi.org/10.1073/pnas.2216734120
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author Xie, Hao
Lyratzakis, Alexandros
Khera, Radhika
Koutantou, Myrto
Welsch, Sonja
Michel, Hartmut
Tsiotis, Georgios
author_facet Xie, Hao
Lyratzakis, Alexandros
Khera, Radhika
Koutantou, Myrto
Welsch, Sonja
Michel, Hartmut
Tsiotis, Georgios
author_sort Xie, Hao
collection PubMed
description Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic electron microscopic structure of the intact FMO-RC apparatus from Chlorobaculum tepidum at 2.5 Å resolution. The FMO-RC apparatus presents an asymmetric architecture and contains two FMO trimers that show different interaction patterns with the RC core. Furthermore, the two permanently bound transmembrane subunits PscC, which donate electrons to the special pair, interact only with the two large PscA subunits. This structure fills an important gap in our understanding of the transfer of energy from antenna to the electron transport chain of this RC and the transfer of electrons from reduced sulfur compounds to the special pair.
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spelling pubmed-99459942023-07-24 Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum Xie, Hao Lyratzakis, Alexandros Khera, Radhika Koutantou, Myrto Welsch, Sonja Michel, Hartmut Tsiotis, Georgios Proc Natl Acad Sci U S A Biological Sciences Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic electron microscopic structure of the intact FMO-RC apparatus from Chlorobaculum tepidum at 2.5 Å resolution. The FMO-RC apparatus presents an asymmetric architecture and contains two FMO trimers that show different interaction patterns with the RC core. Furthermore, the two permanently bound transmembrane subunits PscC, which donate electrons to the special pair, interact only with the two large PscA subunits. This structure fills an important gap in our understanding of the transfer of energy from antenna to the electron transport chain of this RC and the transfer of electrons from reduced sulfur compounds to the special pair. National Academy of Sciences 2023-01-24 2023-01-31 /pmc/articles/PMC9945994/ /pubmed/36693097 http://dx.doi.org/10.1073/pnas.2216734120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Xie, Hao
Lyratzakis, Alexandros
Khera, Radhika
Koutantou, Myrto
Welsch, Sonja
Michel, Hartmut
Tsiotis, Georgios
Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
title Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
title_full Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
title_fullStr Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
title_full_unstemmed Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
title_short Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
title_sort cryo-em structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium chlorobaculum tepidum
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945994/
https://www.ncbi.nlm.nih.gov/pubmed/36693097
http://dx.doi.org/10.1073/pnas.2216734120
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