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Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum
Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945994/ https://www.ncbi.nlm.nih.gov/pubmed/36693097 http://dx.doi.org/10.1073/pnas.2216734120 |
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author | Xie, Hao Lyratzakis, Alexandros Khera, Radhika Koutantou, Myrto Welsch, Sonja Michel, Hartmut Tsiotis, Georgios |
author_facet | Xie, Hao Lyratzakis, Alexandros Khera, Radhika Koutantou, Myrto Welsch, Sonja Michel, Hartmut Tsiotis, Georgios |
author_sort | Xie, Hao |
collection | PubMed |
description | Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic electron microscopic structure of the intact FMO-RC apparatus from Chlorobaculum tepidum at 2.5 Å resolution. The FMO-RC apparatus presents an asymmetric architecture and contains two FMO trimers that show different interaction patterns with the RC core. Furthermore, the two permanently bound transmembrane subunits PscC, which donate electrons to the special pair, interact only with the two large PscA subunits. This structure fills an important gap in our understanding of the transfer of energy from antenna to the electron transport chain of this RC and the transfer of electrons from reduced sulfur compounds to the special pair. |
format | Online Article Text |
id | pubmed-9945994 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-99459942023-07-24 Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum Xie, Hao Lyratzakis, Alexandros Khera, Radhika Koutantou, Myrto Welsch, Sonja Michel, Hartmut Tsiotis, Georgios Proc Natl Acad Sci U S A Biological Sciences Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna–Matthews–Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic electron microscopic structure of the intact FMO-RC apparatus from Chlorobaculum tepidum at 2.5 Å resolution. The FMO-RC apparatus presents an asymmetric architecture and contains two FMO trimers that show different interaction patterns with the RC core. Furthermore, the two permanently bound transmembrane subunits PscC, which donate electrons to the special pair, interact only with the two large PscA subunits. This structure fills an important gap in our understanding of the transfer of energy from antenna to the electron transport chain of this RC and the transfer of electrons from reduced sulfur compounds to the special pair. National Academy of Sciences 2023-01-24 2023-01-31 /pmc/articles/PMC9945994/ /pubmed/36693097 http://dx.doi.org/10.1073/pnas.2216734120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Xie, Hao Lyratzakis, Alexandros Khera, Radhika Koutantou, Myrto Welsch, Sonja Michel, Hartmut Tsiotis, Georgios Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum |
title | Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum |
title_full | Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum |
title_fullStr | Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum |
title_full_unstemmed | Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum |
title_short | Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum |
title_sort | cryo-em structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium chlorobaculum tepidum |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9945994/ https://www.ncbi.nlm.nih.gov/pubmed/36693097 http://dx.doi.org/10.1073/pnas.2216734120 |
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