Cargando…

A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings

In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial inf...

Descripción completa

Detalles Bibliográficos
Autores principales: Gallingani, Tommaso, Resca, Elisa, Dominici, Massimo, Gavioli, Giuliana, Laurita, Romolo, Liguori, Anna, Mari, Giorgio, Ortolani, Luca, Pericolini, Eva, Sala, Arianna, Laghi, Giulia, Petrachi, Tiziana, Arnauld, Gaëlle Francoise, Accorsi, Luca, Rizzoli, Rita, Colombo, Vittorio, Gherardi, Matteo, Veronesi, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946233/
https://www.ncbi.nlm.nih.gov/pubmed/36812218
http://dx.doi.org/10.1371/journal.pone.0282059
_version_ 1784892289708982272
author Gallingani, Tommaso
Resca, Elisa
Dominici, Massimo
Gavioli, Giuliana
Laurita, Romolo
Liguori, Anna
Mari, Giorgio
Ortolani, Luca
Pericolini, Eva
Sala, Arianna
Laghi, Giulia
Petrachi, Tiziana
Arnauld, Gaëlle Francoise
Accorsi, Luca
Rizzoli, Rita
Colombo, Vittorio
Gherardi, Matteo
Veronesi, Elena
author_facet Gallingani, Tommaso
Resca, Elisa
Dominici, Massimo
Gavioli, Giuliana
Laurita, Romolo
Liguori, Anna
Mari, Giorgio
Ortolani, Luca
Pericolini, Eva
Sala, Arianna
Laghi, Giulia
Petrachi, Tiziana
Arnauld, Gaëlle Francoise
Accorsi, Luca
Rizzoli, Rita
Colombo, Vittorio
Gherardi, Matteo
Veronesi, Elena
author_sort Gallingani, Tommaso
collection PubMed
description In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial infection rate without side effects and the development of antibiotic resistance. Beside nosocomial infections, clot formation affects cardiovascular medical devices and central venous catheters implants. In order to reduce and prevent such infection, we develop a plasma-assisted process for the deposition of nanostructured functional coatings on flat substrates and mini catheters. Silver nanoparticles (Ag NPs) are synthesized exploiting in-flight plasma-droplet reactions and are embedded in an organic coating deposited through hexamethyldisiloxane (HMDSO) plasma assisted polymerization. Coating stability upon liquid immersion and ethylene oxide (EtO) sterilization is assessed through chemical and morphological analysis carried out by means of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). In the perspective of future clinical application, an in vitro analysis of anti-biofilm effect has been done. Moreover, we employed a murine model of catheter-associated infection which further highlighted the performance of Ag nanostructured films in counteract biofilm formation. The anti-clot performances coupled by haemo- and cytocompatibility assays have also been performed.
format Online
Article
Text
id pubmed-9946233
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-99462332023-02-23 A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings Gallingani, Tommaso Resca, Elisa Dominici, Massimo Gavioli, Giuliana Laurita, Romolo Liguori, Anna Mari, Giorgio Ortolani, Luca Pericolini, Eva Sala, Arianna Laghi, Giulia Petrachi, Tiziana Arnauld, Gaëlle Francoise Accorsi, Luca Rizzoli, Rita Colombo, Vittorio Gherardi, Matteo Veronesi, Elena PLoS One Research Article In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial infection rate without side effects and the development of antibiotic resistance. Beside nosocomial infections, clot formation affects cardiovascular medical devices and central venous catheters implants. In order to reduce and prevent such infection, we develop a plasma-assisted process for the deposition of nanostructured functional coatings on flat substrates and mini catheters. Silver nanoparticles (Ag NPs) are synthesized exploiting in-flight plasma-droplet reactions and are embedded in an organic coating deposited through hexamethyldisiloxane (HMDSO) plasma assisted polymerization. Coating stability upon liquid immersion and ethylene oxide (EtO) sterilization is assessed through chemical and morphological analysis carried out by means of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). In the perspective of future clinical application, an in vitro analysis of anti-biofilm effect has been done. Moreover, we employed a murine model of catheter-associated infection which further highlighted the performance of Ag nanostructured films in counteract biofilm formation. The anti-clot performances coupled by haemo- and cytocompatibility assays have also been performed. Public Library of Science 2023-02-22 /pmc/articles/PMC9946233/ /pubmed/36812218 http://dx.doi.org/10.1371/journal.pone.0282059 Text en © 2023 Gallingani et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Gallingani, Tommaso
Resca, Elisa
Dominici, Massimo
Gavioli, Giuliana
Laurita, Romolo
Liguori, Anna
Mari, Giorgio
Ortolani, Luca
Pericolini, Eva
Sala, Arianna
Laghi, Giulia
Petrachi, Tiziana
Arnauld, Gaëlle Francoise
Accorsi, Luca
Rizzoli, Rita
Colombo, Vittorio
Gherardi, Matteo
Veronesi, Elena
A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
title A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
title_full A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
title_fullStr A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
title_full_unstemmed A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
title_short A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
title_sort new strategy to prevent biofilm and clot formation in medical devices: the use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946233/
https://www.ncbi.nlm.nih.gov/pubmed/36812218
http://dx.doi.org/10.1371/journal.pone.0282059
work_keys_str_mv AT gallinganitommaso anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT rescaelisa anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT dominicimassimo anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT gavioligiuliana anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT lauritaromolo anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT liguorianna anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT marigiorgio anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT ortolaniluca anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT pericolinieva anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT salaarianna anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT laghigiulia anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT petrachitiziana anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT arnauldgaellefrancoise anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT accorsiluca anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT rizzolirita anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT colombovittorio anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT gherardimatteo anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT veronesielena anewstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT gallinganitommaso newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT rescaelisa newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT dominicimassimo newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT gavioligiuliana newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT lauritaromolo newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT liguorianna newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT marigiorgio newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT ortolaniluca newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT pericolinieva newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT salaarianna newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT laghigiulia newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT petrachitiziana newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT arnauldgaellefrancoise newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT accorsiluca newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT rizzolirita newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT colombovittorio newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT gherardimatteo newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings
AT veronesielena newstrategytopreventbiofilmandclotformationinmedicaldevicestheuseofatmosphericnonthermalplasmaassisteddepositionofsilverbasednanostructuredcoatings