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A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings
In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial inf...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946233/ https://www.ncbi.nlm.nih.gov/pubmed/36812218 http://dx.doi.org/10.1371/journal.pone.0282059 |
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author | Gallingani, Tommaso Resca, Elisa Dominici, Massimo Gavioli, Giuliana Laurita, Romolo Liguori, Anna Mari, Giorgio Ortolani, Luca Pericolini, Eva Sala, Arianna Laghi, Giulia Petrachi, Tiziana Arnauld, Gaëlle Francoise Accorsi, Luca Rizzoli, Rita Colombo, Vittorio Gherardi, Matteo Veronesi, Elena |
author_facet | Gallingani, Tommaso Resca, Elisa Dominici, Massimo Gavioli, Giuliana Laurita, Romolo Liguori, Anna Mari, Giorgio Ortolani, Luca Pericolini, Eva Sala, Arianna Laghi, Giulia Petrachi, Tiziana Arnauld, Gaëlle Francoise Accorsi, Luca Rizzoli, Rita Colombo, Vittorio Gherardi, Matteo Veronesi, Elena |
author_sort | Gallingani, Tommaso |
collection | PubMed |
description | In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial infection rate without side effects and the development of antibiotic resistance. Beside nosocomial infections, clot formation affects cardiovascular medical devices and central venous catheters implants. In order to reduce and prevent such infection, we develop a plasma-assisted process for the deposition of nanostructured functional coatings on flat substrates and mini catheters. Silver nanoparticles (Ag NPs) are synthesized exploiting in-flight plasma-droplet reactions and are embedded in an organic coating deposited through hexamethyldisiloxane (HMDSO) plasma assisted polymerization. Coating stability upon liquid immersion and ethylene oxide (EtO) sterilization is assessed through chemical and morphological analysis carried out by means of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). In the perspective of future clinical application, an in vitro analysis of anti-biofilm effect has been done. Moreover, we employed a murine model of catheter-associated infection which further highlighted the performance of Ag nanostructured films in counteract biofilm formation. The anti-clot performances coupled by haemo- and cytocompatibility assays have also been performed. |
format | Online Article Text |
id | pubmed-9946233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-99462332023-02-23 A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings Gallingani, Tommaso Resca, Elisa Dominici, Massimo Gavioli, Giuliana Laurita, Romolo Liguori, Anna Mari, Giorgio Ortolani, Luca Pericolini, Eva Sala, Arianna Laghi, Giulia Petrachi, Tiziana Arnauld, Gaëlle Francoise Accorsi, Luca Rizzoli, Rita Colombo, Vittorio Gherardi, Matteo Veronesi, Elena PLoS One Research Article In industrialized countries, health care associated infections, the fourth leading cause of disease, are a major health issue. At least half of all cases of nosocomial infections are associated with medical devices. Antibacterial coatings arise as an important approach to restrict the nosocomial infection rate without side effects and the development of antibiotic resistance. Beside nosocomial infections, clot formation affects cardiovascular medical devices and central venous catheters implants. In order to reduce and prevent such infection, we develop a plasma-assisted process for the deposition of nanostructured functional coatings on flat substrates and mini catheters. Silver nanoparticles (Ag NPs) are synthesized exploiting in-flight plasma-droplet reactions and are embedded in an organic coating deposited through hexamethyldisiloxane (HMDSO) plasma assisted polymerization. Coating stability upon liquid immersion and ethylene oxide (EtO) sterilization is assessed through chemical and morphological analysis carried out by means of Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). In the perspective of future clinical application, an in vitro analysis of anti-biofilm effect has been done. Moreover, we employed a murine model of catheter-associated infection which further highlighted the performance of Ag nanostructured films in counteract biofilm formation. The anti-clot performances coupled by haemo- and cytocompatibility assays have also been performed. Public Library of Science 2023-02-22 /pmc/articles/PMC9946233/ /pubmed/36812218 http://dx.doi.org/10.1371/journal.pone.0282059 Text en © 2023 Gallingani et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Gallingani, Tommaso Resca, Elisa Dominici, Massimo Gavioli, Giuliana Laurita, Romolo Liguori, Anna Mari, Giorgio Ortolani, Luca Pericolini, Eva Sala, Arianna Laghi, Giulia Petrachi, Tiziana Arnauld, Gaëlle Francoise Accorsi, Luca Rizzoli, Rita Colombo, Vittorio Gherardi, Matteo Veronesi, Elena A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
title | A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
title_full | A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
title_fullStr | A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
title_full_unstemmed | A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
title_short | A new strategy to prevent biofilm and clot formation in medical devices: The use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
title_sort | new strategy to prevent biofilm and clot formation in medical devices: the use of atmospheric non-thermal plasma assisted deposition of silver-based nanostructured coatings |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946233/ https://www.ncbi.nlm.nih.gov/pubmed/36812218 http://dx.doi.org/10.1371/journal.pone.0282059 |
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