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Biomineralogical signatures of breast microcalcifications

Microcalcifications, primarily biogenic apatite, occur in cancerous and benign breast pathologies and are key mammographic indicators. Outside the clinic, numerous microcalcification compositional metrics (e.g., carbonate and metal content) are linked to malignancy, yet microcalcification formation...

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Autores principales: Kunitake, Jennie A. M. R., Sudilovsky, Daniel, Johnson, Lynn M., Loh, Hyun-Chae, Choi, Siyoung, Morris, Patrick G., Jochelson, Maxine S., Iyengar, Neil M., Morrow, Monica, Masic, Admir, Fischbach, Claudia, Estroff, Lara A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946357/
https://www.ncbi.nlm.nih.gov/pubmed/36812311
http://dx.doi.org/10.1126/sciadv.ade3152
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author Kunitake, Jennie A. M. R.
Sudilovsky, Daniel
Johnson, Lynn M.
Loh, Hyun-Chae
Choi, Siyoung
Morris, Patrick G.
Jochelson, Maxine S.
Iyengar, Neil M.
Morrow, Monica
Masic, Admir
Fischbach, Claudia
Estroff, Lara A.
author_facet Kunitake, Jennie A. M. R.
Sudilovsky, Daniel
Johnson, Lynn M.
Loh, Hyun-Chae
Choi, Siyoung
Morris, Patrick G.
Jochelson, Maxine S.
Iyengar, Neil M.
Morrow, Monica
Masic, Admir
Fischbach, Claudia
Estroff, Lara A.
author_sort Kunitake, Jennie A. M. R.
collection PubMed
description Microcalcifications, primarily biogenic apatite, occur in cancerous and benign breast pathologies and are key mammographic indicators. Outside the clinic, numerous microcalcification compositional metrics (e.g., carbonate and metal content) are linked to malignancy, yet microcalcification formation is dependent on microenvironmental conditions, which are notoriously heterogeneous in breast cancer. We interrogate multiscale heterogeneity in 93 calcifications from 21 breast cancer patients using an omics-inspired approach: For each microcalcification, we define a “biomineralogical signature” combining metrics derived from Raman microscopy and energy-dispersive spectroscopy. We observe that (i) calcifications cluster into physiologically relevant groups reflecting tissue type and local malignancy; (ii) carbonate content exhibits substantial intratumor heterogeneity; (iii) trace metals including zinc, iron, and aluminum are enhanced in malignant-localized calcifications; and (iv) the lipid-to-protein ratio within calcifications is lower in patients with poor composite outcome, suggesting that there is potential clinical value in expanding research on calcification diagnostic metrics to include “mineral-entrapped” organic matrix.
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spelling pubmed-99463572023-02-23 Biomineralogical signatures of breast microcalcifications Kunitake, Jennie A. M. R. Sudilovsky, Daniel Johnson, Lynn M. Loh, Hyun-Chae Choi, Siyoung Morris, Patrick G. Jochelson, Maxine S. Iyengar, Neil M. Morrow, Monica Masic, Admir Fischbach, Claudia Estroff, Lara A. Sci Adv Biomedicine and Life Sciences Microcalcifications, primarily biogenic apatite, occur in cancerous and benign breast pathologies and are key mammographic indicators. Outside the clinic, numerous microcalcification compositional metrics (e.g., carbonate and metal content) are linked to malignancy, yet microcalcification formation is dependent on microenvironmental conditions, which are notoriously heterogeneous in breast cancer. We interrogate multiscale heterogeneity in 93 calcifications from 21 breast cancer patients using an omics-inspired approach: For each microcalcification, we define a “biomineralogical signature” combining metrics derived from Raman microscopy and energy-dispersive spectroscopy. We observe that (i) calcifications cluster into physiologically relevant groups reflecting tissue type and local malignancy; (ii) carbonate content exhibits substantial intratumor heterogeneity; (iii) trace metals including zinc, iron, and aluminum are enhanced in malignant-localized calcifications; and (iv) the lipid-to-protein ratio within calcifications is lower in patients with poor composite outcome, suggesting that there is potential clinical value in expanding research on calcification diagnostic metrics to include “mineral-entrapped” organic matrix. American Association for the Advancement of Science 2023-02-22 /pmc/articles/PMC9946357/ /pubmed/36812311 http://dx.doi.org/10.1126/sciadv.ade3152 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Kunitake, Jennie A. M. R.
Sudilovsky, Daniel
Johnson, Lynn M.
Loh, Hyun-Chae
Choi, Siyoung
Morris, Patrick G.
Jochelson, Maxine S.
Iyengar, Neil M.
Morrow, Monica
Masic, Admir
Fischbach, Claudia
Estroff, Lara A.
Biomineralogical signatures of breast microcalcifications
title Biomineralogical signatures of breast microcalcifications
title_full Biomineralogical signatures of breast microcalcifications
title_fullStr Biomineralogical signatures of breast microcalcifications
title_full_unstemmed Biomineralogical signatures of breast microcalcifications
title_short Biomineralogical signatures of breast microcalcifications
title_sort biomineralogical signatures of breast microcalcifications
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946357/
https://www.ncbi.nlm.nih.gov/pubmed/36812311
http://dx.doi.org/10.1126/sciadv.ade3152
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