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A single-cell atlas of murine reproductive tissues during preterm labor
Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from the syndrome of preterm labor. The best-established causal link to preterm labor is intra-amniotic infection, which involves premature activation of the parturition cascade in the reproductive ti...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946687/ https://www.ncbi.nlm.nih.gov/pubmed/36599348 http://dx.doi.org/10.1016/j.celrep.2022.111846 |
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author | Garcia-Flores, Valeria Romero, Roberto Peyvandipour, Azam Galaz, Jose Pusod, Errile Panaitescu, Bogdan Miller, Derek Xu, Yi Tao, Li Liu, Zhenjie Tarca, Adi L. Pique-Regi, Roger Gomez-Lopez, Nardhy |
author_facet | Garcia-Flores, Valeria Romero, Roberto Peyvandipour, Azam Galaz, Jose Pusod, Errile Panaitescu, Bogdan Miller, Derek Xu, Yi Tao, Li Liu, Zhenjie Tarca, Adi L. Pique-Regi, Roger Gomez-Lopez, Nardhy |
author_sort | Garcia-Flores, Valeria |
collection | PubMed |
description | Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from the syndrome of preterm labor. The best-established causal link to preterm labor is intra-amniotic infection, which involves premature activation of the parturition cascade in the reproductive tissues. Herein, we utilize single-cell RNA sequencing (scRNA-seq) to generate a single-cell atlas of the murine uterus, decidua, and cervix in a model of infection-induced preterm labor. We show that preterm labor affects the transcriptomic profiles of specific immune and non-immune cell subsets. Shared and tissue-specific gene expression signatures are identified among affected cells. Determination of intercellular communications implicates specific cell types in preterm labor-associated signaling pathways across tissues. In silico comparison of murine and human uterine cell-cell interactions reveals conserved signaling pathways implicated in labor. Thus, our scRNA-seq data provide insights into the preterm labor-driven cellular landscape and communications in reproductive tissues. |
format | Online Article Text |
id | pubmed-9946687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
record_format | MEDLINE/PubMed |
spelling | pubmed-99466872023-02-22 A single-cell atlas of murine reproductive tissues during preterm labor Garcia-Flores, Valeria Romero, Roberto Peyvandipour, Azam Galaz, Jose Pusod, Errile Panaitescu, Bogdan Miller, Derek Xu, Yi Tao, Li Liu, Zhenjie Tarca, Adi L. Pique-Regi, Roger Gomez-Lopez, Nardhy Cell Rep Article Preterm birth, the leading cause of perinatal morbidity and mortality worldwide, frequently results from the syndrome of preterm labor. The best-established causal link to preterm labor is intra-amniotic infection, which involves premature activation of the parturition cascade in the reproductive tissues. Herein, we utilize single-cell RNA sequencing (scRNA-seq) to generate a single-cell atlas of the murine uterus, decidua, and cervix in a model of infection-induced preterm labor. We show that preterm labor affects the transcriptomic profiles of specific immune and non-immune cell subsets. Shared and tissue-specific gene expression signatures are identified among affected cells. Determination of intercellular communications implicates specific cell types in preterm labor-associated signaling pathways across tissues. In silico comparison of murine and human uterine cell-cell interactions reveals conserved signaling pathways implicated in labor. Thus, our scRNA-seq data provide insights into the preterm labor-driven cellular landscape and communications in reproductive tissues. 2023-01-31 2023-01-03 /pmc/articles/PMC9946687/ /pubmed/36599348 http://dx.doi.org/10.1016/j.celrep.2022.111846 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Garcia-Flores, Valeria Romero, Roberto Peyvandipour, Azam Galaz, Jose Pusod, Errile Panaitescu, Bogdan Miller, Derek Xu, Yi Tao, Li Liu, Zhenjie Tarca, Adi L. Pique-Regi, Roger Gomez-Lopez, Nardhy A single-cell atlas of murine reproductive tissues during preterm labor |
title | A single-cell atlas of murine reproductive tissues during preterm labor |
title_full | A single-cell atlas of murine reproductive tissues during preterm labor |
title_fullStr | A single-cell atlas of murine reproductive tissues during preterm labor |
title_full_unstemmed | A single-cell atlas of murine reproductive tissues during preterm labor |
title_short | A single-cell atlas of murine reproductive tissues during preterm labor |
title_sort | single-cell atlas of murine reproductive tissues during preterm labor |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946687/ https://www.ncbi.nlm.nih.gov/pubmed/36599348 http://dx.doi.org/10.1016/j.celrep.2022.111846 |
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