Protocol for bulk RNA sequencing of enriched human neutrophils from whole blood and estimation of sample purity

Although neutrophils are the most abundant leukocyte in healthy individuals and impact outcomes of diseases ranging from sepsis to cancer, they remain understudied due to technical constraints of isolation, preservation, and sequencing. We present a modified Smart-Seq2 protocol for bulk RNA sequenci...

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Detalles Bibliográficos
Autores principales: Gonye, Anna L.K., LaSalle, Thomas J., Freeman, Samuel S., Reyes, Miguel, Hacohen, Nir, Villani, Alexandra-Chloé, Sade-Feldman, Moshe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946790/
https://www.ncbi.nlm.nih.gov/pubmed/36853705
http://dx.doi.org/10.1016/j.xpro.2023.102125

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946790/
https://www.ncbi.nlm.nih.gov/pubmed/36853705
http://dx.doi.org/10.1016/j.xpro.2023.102125

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