Cargando…
Protocol for bulk RNA sequencing of enriched human neutrophils from whole blood and estimation of sample purity
Although neutrophils are the most abundant leukocyte in healthy individuals and impact outcomes of diseases ranging from sepsis to cancer, they remain understudied due to technical constraints of isolation, preservation, and sequencing. We present a modified Smart-Seq2 protocol for bulk RNA sequenci...
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9946790/ https://www.ncbi.nlm.nih.gov/pubmed/36853705 http://dx.doi.org/10.1016/j.xpro.2023.102125 |